| 细胞周期素D2启动子曲古菌素A效应区域的研究 |
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| 引用本文： | 郭志义,郝小惠,田炜,谭菲菲,姜雪莲,李潇婷,胡倩倩,杨方. 细胞周期素D2启动子曲古菌素A效应区域的研究[J]. 第三军医大学学报, 2011, 33(14): 1458-1461 |
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| 作者姓名： | 郭志义 郝小惠 田炜 谭菲菲 姜雪莲 李潇婷 胡倩倩 杨方 |
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| 作者单位： | 1. 唐山,河北联合大学,医学实验研究中心,河北省煤矿卫生与安全重点实验室,河北,063000
2. 唐山,河北联合大学,生命科学院,河北,063000 |
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| 基金项目： | 国家自然科学基金(31050010); 河北省教育厅青年基金(2010154); 华北煤炭医学院硕博启动基金(BS06002)~~ |
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| 摘 要： | 目的 研究在曲古菌素A(trichostatin A,TSA)处理条件下细胞周期素D2的调控机制.方法 细胞周期素D2的mRNA水平通过RT-PCR方法 检测.细胞周期素D2的启动子片段以A549细胞基因组DNA为模板,通过高保真PCR扩增获得,并克隆到pGL3-BASIC载体中;并以此为基础通过高保真PCR扩增出不同...
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| 关 键 词： | 细胞周期素D2 启动子 报告基因 曲古菌素A |
Determination of cyclin D_2 promoter region in response to trichostatin A treatment |
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| Guo Zhiyi,Hao Xiaohui,Tian Wei,Tan Feifei,Jiang Xuelian,Li Xiaoting,Hu Qianqian,Yang Fang. Determination of cyclin D_2 promoter region in response to trichostatin A treatment[J]. Acta Academiae Medicinae Militaris Tertiae, 2011, 33(14): 1458-1461 |
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| Authors: | Guo Zhiyi Hao Xiaohui Tian Wei Tan Feifei Jiang Xuelian Li Xiaoting Hu Qianqian Yang Fang |
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| Affiliation: | Guo Zhiyi1,Hao Xiaohui2,Tian Wei1,Tan Feifei2,Jiang Xuelian2,Li Xiaoting2,Hu Qianqian2,Yang Fang1(1Hebei Province Key Laboratory of Occupational Health and Safety for Coal Industry,Medical Research Center,2 School of Life Science,Hebei United University,Tangshan,Hebei Province,063000,China) |
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| Abstract: | Objective To explore the regulation mechanism of cyclin D2 under trichostatin A(TSA) treatment.Methods The mRNA level of cyclin D2 was detected by RT-PCR.Cyclin D2 promoter fragment was amplified by high-fidelity PCR with A549 cell genome DNA as a template,and was then inserted into plasmid vector pGL3-BASIC.Truncated promoter fragments were amplified based on the recombinant plasmid.The relative activity of the promoter fragments was evaluated with the firefly luciferase reporter gene method.Results The mR... |
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| Keywords: | cyclin D2 promoter reporter gene trichostatin A |
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