关节镜下半月板部分切除制备骨关节炎动物模型

目的 探讨经关节镜建立猪双膝骨关节炎动物模型及用磁共振成像(magnetic resonance imaging, MRI)监测骨关节炎骨软骨损害的有效性和可行性.方法 4只小型猪经关节镜切除双膝外侧部分半月板,术后12周,行大体、组织学和MRI观察与切除半月板相对股骨髁的骨软骨变化.结果 大体观察结果显示,局限性软骨表面纤维化,股骨髁间切迹骨赘形成;组织学检测结果显示,软骨细胞数量和蛋白多糖含量减少、成簇排列细胞数量增加;MRI检查结果显示,软骨厚度变薄和骨软骨信号强度改变.结论 经关节镜半月板部分切除能有效建立猪双膝关节骨关节炎动物模型,MRI能灵敏地监测到骨关节炎动物模型的骨软骨变化.

Establishment of animal models of bilateral knee joint osteoarthritis by arthroscopy

Objective To explore the effectiveness and the feasibility of establishment of osteoarthritis animal model by arthroscopy and monitor the osteoarthritis lesion of bone and cartilage by magnetic resonance imaging (MRI) in bilateral knee joints of mini-pigs. Methods Bilateral partial menisci resections were performed on four mini-pigs (8 joints) by arthroscopy. Changes of bone and cartilage contacted with the resected menisci were analyzed by gross observation, histology, and MRI at 12 weeks after surgery. Results Gross observation showed focal fibrillation on articular cartilage surfaces and significant osteophyte formation at lateral intercondylar notches of the distal femur. Histology revealed the loss of cellularity and proteoglycan and increase in the number of clustering-arrayed chondrocytes. MRI showed the cartilage thickness was decreased and signal intensity of bone and cartilage changed. Conclusion Osteoarthritis animal models in bilateral knee joints of mini-pigs can be established effectively by partial meniscectomy under arthroscope. Changes of bone and cartilage of osteoarthritis animal models can be monitored sensitively by MRI.