427株细菌L型医院感染与耐药分析

目的 为提高医院感染细菌培养阳性率及抗菌药物的有效作用。方法 对我院１９９５年１月－１９９７年１２月从１８７０例住院患者采取血，尿，脑脊液，胸腹水，胆汁等标本进行了常规与高渗同步培养的结果进行了对照分析。结果 分离出Ｌ型细菌４２７株，而普通培养均为阴性，阳性率提高了２２．８％，分离菌种达１１种之多；以金黄色葡萄球菌在各类标本中感染率占首位，１９．４４％；大肠埃希菌占第２位，１６．４％，凝固酶阴性葡

Analysison 427 strains of bacteria L-form in nosocomial infection and the drug resistance

OBJECTIVE Toenhance the positive culture rate of bacteria in nosoconial infection and to guide theproper use of antibiotics.METHODS The routine and hyperosmotic culture results performedon the samples of blood ,urine,cerebrospinal fluid,pleural effusion,ascites and bile from1870 inpatients during Jan 1995 to Dec 1997 were analyzed in tandem.RESULTS 427 strains ofbacteria L-form were separated out with the synchronous culture method from the negativespecimens on routine culture, the positive rate was increased by 22.8%.The posifivestrains were belonged to 11 kinds of bacteria, in which the first was S.aureus,the secondwas E.coil,and then coagulase-negative Staphylococcus,Enterococcus D group and yeast.Their positive rates were 19.44%,16.4%,11%,10.77%and 10.3% respectively.The drugsensitivity results showed that they were highly resistant to penicillin andcephalosporins,lower to erythromycin,chloramphenical, gentamicin and lincomycin.CONCLUSIONS The method step by step culture may not only increase the positive culturerate,but also provide very important clew in the diagnosis of doubtful cases and enhancethe rational administration of antibiotics.