大肠癌EGFR、HER-2、VEGF表达特点及其对分子靶向治疗的指导意义

目的:探讨大肠癌中表皮生长因子受体(EGFR)、人类表皮生长因子受体-2(HER-2)和血管内皮生长因子(VEGF)的表达特点及其对大肠癌分子靶向治疗的指导意义.方法:随机选取2005-05/2009-03中国人民解放军空军总医院普通外科行根治性手术的大肠癌患者78例. 应用免疫组织化学法检测大肠癌肿瘤组织中EGFR、HER-2、VEGF的表达, 并结合其临床病理特点进行回顾性分析.结果:EGF R、HE R-2、VEGF在大肠癌中的阳性表达率依次为38 . 5%( 30 / 78 ) 、53.8%(42/78)、41.0%(32/78). 三者的表达与性别、年龄、肿瘤部位、分化类型无关, 而与肿瘤的大小、侵袭深度和转移密切相关.EGFR与HER-2及VEGF, HER-2与VEGF在大肠癌肿瘤组织中表达呈正相关(r = 0.421,0.484, 0.469, P = 0.019, 0.012, 0.016).结论:EGFR、HER-2、VEGF的表达参与大肠癌的生长、侵袭和转移过程. 三者的联合检测可作为判断大肠癌预后、筛选高危转移患者的有效指标, 同时, 也可用于指导大肠癌的靶向药物治疗.

Expression of EGFR, HER-2,VEGF in tumour tissues and their directive significance for the molecular targeting therapy of colorectal carcinoma

AIM: To detect the characteristics of epidermal growth factor receptor (EGFR),human epidermal receptor-2 (HER-2) and vascular endothelial growth factor (VEGF) expression in colorectal carcinoma (CRC) tumor tissues, and to analyze their directive significance for the molecular targeting therapy of colorectal carcinoma (CRC). METHODS: Seventy eight patients with CRC were randomly selected, who underwent radical surgery in the department of general surgery at our hospital from May 2005 to March 2009. The SP Immunohistochemical staining was used to detect EGFR, HER-2 and VEGF protein expression in 78 CRC tumor tissue samples. And their corresponding clinical data were analysed retrospectively. RESULTS: The expression rates of EGFR, HER-2 and VEGF protein in CRC tumour tissue were 38.5% (30/78), 53.8% (42/78) and 41.0% (32/78). Positive rates of EGFR, HER-2 and VEGF protein were closely relevant to the tumour size, knubbly invasive depth and lymph node metastasis; but not to the patient's sex, age, tumor site or histology grade. There was a stable positive relationship among EGFR, HER-2 and VEGF expression in CRC tumour tissue samples. The related coefficients were 0.421, 0.484 and 0.469 respectively (P = 0.019, 0.012 and 0.016). CONCLUSION: The present study demonstrates that the EGFR, HER-2 and VEGF expression participate in the development, invasion and metastasis process of CRC. Their joint detection can be looked as an important symbol for judging the prognosis of CRC, screening the high-risk metastasis patients and guiding the molecular targeting therapy of colorectal carcinoma.