| 不同方法提取益智挥发油后水溶性成分的比较 |
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| 引用本文： | 李泽友,吴娇,王勇,刘明生. 不同方法提取益智挥发油后水溶性成分的比较[J]. 海南医学院学报, 2009, 15(12): 1498-1500 |
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| 作者姓名： | 李泽友 吴娇 王勇 刘明生 |
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| 作者单位： | 1. 海南医学院药学系
2. 海南医学院2005级本科生,海南,海口,571101 |
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| 基金项目： | 海南省教育厅高等学校科学研究指导性项目 |
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| 摘 要： | 目的：对超临界CO2萃取与水蒸气蒸馏提取益智挥发油后的水溶性成分进行比较。方法：采用高效液相色谱法,以Diamonsil C18色谱柱（250mm×4.6mm,5μm）为固定相,甲醇-水为流动相梯度洗脱,PAD检测器,检测波长254nm,流速1.0mL/min,柱温30℃。结果：2种方法提取挥发油后的水溶性成分都较多且谱图比较相似。结论：益智临床使用时应考虑到传统用法而尽可能保留非挥发性成分。
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| 关 键 词： | 益智 挥发油 水溶性成分 高效液相色谱 |
Comparision of water soluble components in Alpinia oxyphylla after volatile oil extraction |
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| LI Ze-you,WU Jiao,WANG Yong,LIU Ming-Sheng. Comparision of water soluble components in Alpinia oxyphylla after volatile oil extraction[J]. Journal of Hainan Medical College, 2009, 15(12): 1498-1500 |
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| Authors: | LI Ze-you WU Jiao WANG Yong LIU Ming-Sheng |
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| Affiliation: | LI Ze-you, WU Jiao, WANG Yong, LIU Ming-Sheng (1. Pharmacology Department;2. Undergraduate at Grade 2005, Hainan Medical College Haikou 571101 ,China) |
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| Abstract: | Objective:To compare water soluble components in Alpinia oxyphylla after volatile oil extraction with supercritical CO2 extraction technology and steam distillation technology.Methods: Using a high performance liquid chromatography (HPLC) method with Diamonsil C18(4.6 mm×250 mm,5μm)column,methanol-water was gradient-eluted in mobile phase.The detection wavelength was at 254 nm with PAD detector,flow rate was 1.0 mL/min,and column temperature was 30℃.Results: The water soluble components in Alpinia oxyphylla after volatile oil extraction with these two methods were various with similar spectrograms.Conclusion: The traditional usage must be considered and the non-volatile components of Alpinia oxyphylla must be kept as far as possible in clinic application. |
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| Keywords: | Alpinia oxyphylla Water soluble component HPLC |
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