螺旋霉素发酵代谢物的分析及其对产物合成的影响

本文利用膜技术在螺旋霉素(SPM)发酵过程中让发酵液中的某些成份通过膜渗透到透析液中,然后用离子色谱分析仪、纸层析和氨基酸分析仪分析透析液中的代谢产物。经711阴离子交换树脂分离,发现洗脱液中含有乙酸、丙酸、丁酸、丙酮酸和草酰乙酸。用732强酸性阴离子树脂和纸层析初步分离和分析了透析液中天冬酰胺和谷氨酰胺的含量;经氨基酸分析仪测定,透析液中含有15种氨基酸。经静息细胞系统试验,发现短链脂肪酸有促进SPM合成的作用,而谷氨酰胺(1.0mmol/L)、天冬酰胺(2.0mmol/L)会抑制SPM的合成。膜过滤能降低发酵罐内谷氨酰胺和天冬酰胺浓度。这很可能是膜过滤发酵能提高SPM总产率的原因之一。

ANALYSIS OF METABOLITES IN SPIRAMYCIN FERMENTATION AND THEIR EFFECTS ON ANTIBIOTIC BIOSYNTHESIS

Current studies made use of membrane technology to enable certain ingredients of culture broth to penetrate against the membrane into the dialysate during Spiramycin (SPM) fermentation.The dialysa-te was analyzed subsequently with ion chromatography, paper chromato-graphy and amino acid analyzer. By using ammonium form of exchange resin type 7ll, pyruvate, acetate, propionate, butyrate and oxaloacetate were found existing in the dialysate. The concentration of asparagine and glutamine were saparated and determined with strong acidic cation exchange resin and paper chromatography. By using amino acid analyzer, 15 amino acids detected are as follows:asparagine, glutamine, arginine, alanine, cystein, methionine, glutamine, lysine, tyrosine, cystine, valine, pheny-lalanine, isoleucine, leucine and glycine. Among the above mentioned amino acids, asparagine and glutamine are the two highest ones.In order to reveal which components show adverse effect on the biosynthesis, resting cell system experiments were conducted.Results show that short chain fatty acids were favorable to SPM biosynthesis, while high concentration of asparagine and glutamine have negative effect. Due to membrane filtration could retard the cross flow of these metabolites, this is one of the reasons that why SPM fermentation coupled with membrane dialysis could enhance total productivity of SPM.