吡罗昔康对人γδT细胞和消化系统肿瘤细胞的作用比较

目的:探讨吡罗昔康对人γδT细胞杀伤消化系统肿瘤细胞株的影响。方法:用异戊烯焦磷酸法体外扩增人外周血γδT细胞。用不同浓度的吡罗昔康诱导γδT细胞和消化系统肿瘤SGC-7901、SW-1990、SW-480、SW-1116、LOVO细胞株,用MTT法检测吡罗昔康对这些细胞的抑制率和用乳酸脱氢酶法测定γδT细胞的杀伤活性,用流式细胞术检测诱导前后的γδT细胞和SGC-7901、SW-1990、SW-480、SW-1116和LOVO细胞凋亡百分率。结果:γδT细胞培养10d时从扩增前4.21%增加到70.35%。吡罗昔康各种浓度对SGC-7901、SW-1990、SW-480、SW-1116和LOVO细胞株抑制率明显高于γδT细胞。0.01~0.04mmol/L吡罗昔康诱导24h后的γδT细胞对5种肿瘤细胞的杀伤活性最高,浓度超过0.04mmol/L时杀伤活性呈下降趋势;SGC-7901、SW-1990、SW-480、SW-1116和LOVO细胞经不同浓度的吡罗昔康诱导24h后γδT细胞对其的杀伤活性与对照组比较无明显变化。0.16mmol/L吡罗昔康诱导24h对SGC-7901、SW-1990、SW-480、SW-1116和LOVO细胞株的凋亡率分别为12.26%、13.46%、14.59%、25.64%和39.36%,显著高于γδT细胞(8.16%)。结论:吡罗昔康在临床常规使用的药物浓度时可增强γδT细胞杀伤肿瘤细胞作用,超过这一浓度可明显抑制γδT细胞和肿瘤细胞的增殖能力和杀伤活性及增加细胞的凋亡率;吡罗昔康对肿瘤细胞株的抑制率明显高于γδT细胞。这一结果为临床吡罗昔康预防消化道肿瘤的用量提供了实验依据。

A comparison of piroxicam on human γδT cells and digestive tumor cells lines

Objective:To explore the effect of Piroxicam on human γδT cells cytotoxicity and digestive tumor cell lines. Methods: IPP methd was used to amplify human peripheral blood γδT cells in vitro,different concentrations of Piroxicam were used to induce γδT cells and digestive tract tumor cell lines SGC-7901,SW-1990,SW-480,SW-1116 and LOVO,MTT assays is used to detect the inhibition ratio of Piroxicam on these cells, LDH assays is used to measure cytotoxic activity of γδT cells, flow cytometry analysis the apoptosis ratio before and after induced γδT cells and SGC-7901,SW-1990,SW-480,SW-1116 and LOVO.Results: γδT cells were cultured for ten days which proliferation ratio increase from 4.21% to 70.35% , the growth inhibition ratio of different concentrations of Piroxicam on SGC-7901,SW-1990,SW-480,SW-1116 and LOVO were strikingly higher than γδT cells ,when Piroxicam ’s concentration were ranged from 0.01 mmol/L to 0.04 mmol/L,through these various concentration induced γδT cells for 24 hours ,which cytotoxicity against five kinds of tumor cell lines are highest, if piroxicam’s concentration surpass 0.04mmol/L that γδT cells cytotoxic activity present decrease tendency,SGC-7901,SW-1990,SW-480,SW-1116 and LOVO were induced for 24 hours by different concentrations of Piroxicam,compared with control group, γδT cells cytotoxicity tothese cell lines have not marked changed ,when tumor cell lines and γδT cells were induced by piroxicam for 24 hours which concentration is 0.16mmol/L,the apoptosis ratio of tumor cell lines SGC-7901,SW-1990,SW-480,SW-1116 and LOVO are notable higher(respectively 12.26%,13.46%,14.59%,25.64%,39.36% ) than γδT cells (8.16%). Conclusion: piroxicam in clinical routing practical concentration can enhance the effect of γδT cells cytotocity against tumor cells ,and the effect of piroxicam on inhibiting tumor cell lines are clearly higher than γδT cells ,This result may provide an experiment basis for applying piroxicam to prevent digestive tumor in clinical practice.