Skipjack tuna (Katsuwonus pelamis) eyeball oil exerts an anti-inflammatory effect by inhibiting NF-κB and MAPK activation in LPS-induced RAW 264.7 cells and croton oil-treated mice |
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| Institution: | 1. Department of Food Science and Technology, Institute of Food Science, Pukyong National University, Busan 608-737, Republic of Korea;2. Institute of Fisheries Sciences, Pukyong National University, 474, Ilgwang-ro, Ilgwang-myeon, Gijang-gun, Busan 619-911, Republic of Korea;1. Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, 53-114 Wrocław, Poland;2. The Medical University of Silesia, School of Pharmacy with the Division of Laboratory Medicine, Department of Organic Chemistry, Jagiellońska 4, 41-200 Sosnowiec, Poland;1. Department of Rheumatology, Shenzhen Children''s Hospital, Affiliated with Chongqing Medical University, China;2. Department of Rheumatology, Chongqing Children''s Hospital, Affiliated with Chongqing Medical University, China;1. Department of Anesthesiology, West China Hospital of Sichuan University, Chengdu, China;2. Department of Anesthesiology, Affiliated Hospital of Southwest Medical University, Luzhou, China;1. Research Center for Drug Discovery Technology, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea;2. Graduate School of New Drug Discovery and Development, Chungnam National University, Daejeon 34134, Republic of Korea;3. Department of Medicinal and Pharmaceutical Chemistry, University of Science and Technology, 34113 Daejeon, Republic of Korea;4. Research Center for Medicinal Chemistry, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea;1. Laboratory of Biological Cancer Therapy, Cancer Institute, Xuzhou Medical University, Xuzhou 221002, China;2. Jiangsu Center for the Collaboration and Innovation of Cancer Biotherapy, Cancer Institute, Xuzhou Medical University, Xuzhou, Jiangsu 221002, China;3. Department of General Surgery, the Affiliated Hospital of Xuzhou Medical University, Xuzhou Medical University, Xuzhou 221002, China |
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| Abstract: | The effect of tuna eyeball oil (TEO) on lipopolysaccharide (LPS)-induced inflammation in macrophage cells was investigated. TEO had no cytotoxicity in cell viability as compared to the control in LPS induced RAW 264.7 cells. TEO reduced the levels of NO and pro-inflammatory cytokines by up to 50% in a dose-dependent manner. The expression of NF-κB and MAPKs as well as iNOS and COX-2 proteins was reduced by TEO, which suggests that its anti-inflammatory activity is related to the suppression of the NF-κB and MAPK signaling pathways. The rate of formation of ear edema was reduced compared to that in the control at the highest dose tested. In an acute toxicity test, no mice were killed by TEO doses of up to 5000 mg/kg body weight during the two week observation period. These results suggested that TEO may have a significant effect on inflammatory factors and be a potential anti-inflammatory therapeutic. |
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