| 超高效液相色谱-串联质谱测定食品中4种真菌毒素残留量 |
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| 引用本文： | 崔勇,李青,刘思洁,等. 超高效液相色谱-串联质谱测定食品中4种真菌毒素残留量[J]. 中国卫生工程学, 2014, 0(1): 46-48,51 |
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| 作者姓名： | 崔勇 李青 刘思洁 等 |
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| 作者单位： | 吉林省卫生监测检验中心理化检验所,吉林长春130062 |
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| 摘 要： | 目的建立同时检测食品中3-乙酰脱氧雪腐镰刀菌烯醇（3-ADON）、15-乙酰脱氧雪腐镰刀菌烯醇（15-ADON）、雪腐镰刀菌烯醇（NIV）和脱氧雪腐镰刀菌烯醇（DON）的超高效液相色谱一串联质谱（UPLC-MS／Ms）分析方法。方法采用WatersAcquityUPLCBEHC18色谱柱（1．7μm，50mm×2．1mm）、乙腈一0．1％氨水流动相、柱温35℃、质谱采用电喷雾电离（ESI）多反应监测（MRM）负离子模式进行检测，样品以乙腈一水为提取溶剂，经C-18固体萃取吸附剂除去基质干扰，取10μl注入UPLC-MS／MS。结果3-乙酰脱氧雪腐镰刀菌烯醇（3-ADON）、15-乙酰脱氧雪腐镰刀菌烯醇（15-ADON）、雪腐镰刀菌烯醇（NIV）和脱氧雪腐镰刀菌烯醇（DON）在5～200μg／L范围内呈良好的线性关系，加标回收率在85．9％～109．8％，所测定的相对标准偏差在2．34％～5．20％之间。结论该方法灵敏、快速，经济，结果准确可靠，可为食品中4种真菌毒素检测提供可靠的分析方法。
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| 关 键 词： | 食品 真菌毒素 超高效液相色谱-串联质谱 多反应监测 |
Simultaneous determination of four mycotoxins residue in food by ultra performance liquid chromatography- mass/mass spectrometry |
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| Affiliation: | CUI Yong, LI Qing , LIU Si -jie , ZHANG Guan- ying( Physical and Chemical Laboratory, Jilin Provincial Center for Sanitation Inspection and Test, Changchun Jilin 130062, China) |
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| Abstract: | Objective To develop an ultra performance liquid chromatography - mass/mass spectrometry method for the simultaneous determination of 3 - ADON, 15 - ADON, NIV and DON in food. Methods Conditions ： column, Acquity UP- LC BEH C 18 （ 1. 7 Ixm,50 mm × 2. 1 mm） ; mobile phase, acetonitrile - 0. 1% ammonia water; column temperature, 35 ℃ ; The detection was carried on a mass spectrometer coupled with ESI and MRM negative mode. The analytes in food were ex- tracted with water - acetonitrile. The extract was cleaned up using Cls solid phase extraction sorbent, injection volume was 10 μl. Results The linearity of 3 -ADON, 15 -ADON, NIV and DON was good in the range of 5 -200 μg/L The recov- ery of the method was 85.9% - 109. 8% and relative standard deviation was 2. 34% - 5.20%. Conclusion The method is accurate, quick and reliable. |
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| Keywords: | Food Mycotoxin UPLC - MS/MS MRM |
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