基于UPLC-DAD指纹图谱结合化学计量学及6种成分含量测定的桑叶质量研究

[目的]建立超高效液相色谱法(UPLC)-DAD指纹图谱方法,结合化学计量学与多指标含量测定综合评价桑叶质量.[方法]采用Agilent EC-C18(2.1 mm×100 mm,1.7μm),柱温35℃;检测波长分别为290 nm(检测没食子酸甲酯、隐绿原酸)和360 nm(检测芦丁、异槲皮苷、山奈酚-3-O-芸香糖...

Study on the quality of mulberry leaves based on UPLC-DAD fingerprint combined with chemometrics and determination of 6 index components

[Objective]A UPLC-DAD fingerprint method was established to comprehensively evaluate the quality of mulberry leaf combined with chemometrics and multi-index content determination.[Methods]The chromatographic column was Agilent EC-C18 column(2.1 mm×100 mm,1.7μm),and the column temperature was set at 35℃.The detection wavelength were set at 290 nm(for methyl gallate and cryptochlorogenic acid)and 360 nm(for rutin,isoquercitrin,kaempferol-3-O-rutinoside,astragalin).The mobile phase was composed of acetonitrile-0.2%phosphate acid solution in gradient elution manner at a flow rate of 0.2 mL/min and Sample volume 2μL.Establish UPLC-DAD fingerprints of 22 batches of mulberry leaf medicinal materials,using cluster analysis and principal component analysis and other chemometric methods,as well as methyl gallate,cryptochlorogenic acid,rutin,isoquercitrin,kaempferol-3-O-rutinoside and astragalus glycosides were used to evaluate the quality of mulberry leaves.[Results]Twenty-two batches of mulberry leaves UPLC-DAD fingerprints confirmed 16 common peaks,the similarities of mulberry leaf medicinal materials from different batches and the control fingerprints are quite different.And 6 shared peaks were identified by the reference substance,the results showed that the separation of the 6 index components was good,and each component had a wide linear range and a good linear relationship(r2>0.999),with good precision,stability,repeatability and sample recovery.[Conclusion]Using UPLC-DAD technology to establish a fingerprint spectrum combined with 6 index component content determination method is convenient,accurate and reproducible,which could be used for quality evaluation of mulberry leaves.