PEDF对肺鳞状细胞癌SK-MES-1细胞增殖、侵袭和凋亡的影响

目的: 探讨色素上皮衍生因子(PEDF)对肺鳞状细胞癌(鳞癌)SK-MES-1细胞增殖、凋亡和侵袭的影响,阐明PEDF与肺鳞癌发生发展的关系。方法: 培养SK-MES-1细胞,设不加PEDF的细胞为阴性对照组,PEDF干预组细胞分别加入180、360和720 nmol·L^-1 PEDF;MTT法检测各组SK-MES-1细胞增殖抑制率,细胞侵袭实验测定阴性对照组和720 nmol·L^-1 PEDF干预组SK-MES-1细胞穿透数,倒置显微镜观察各组SK-MES-1细胞形态学,流式细胞术检测各组SK-MES-1细胞凋亡率。结果: PEDF干预组细胞增殖抑制率均显著高于阴性对照组(P<0.01),不同剂量PEDF干预组间比较差异也有统计学意义(P<0.05)。阴性对照组细胞贴壁生长良好,PEDF干预组细胞排列逐渐稀疏,形态改变明显。720 nmol·L^-1 PEDF干预组SK-MES-1细胞穿透数(31.6±15.8)低于阴性对照组(75.4±19.3),差异有统计学意义(P<0.01)。随着PEDF干预剂量增加,细胞凋亡率逐渐升高,不同剂量PEDF干预组与阴性对照组比较差异均有统计学意义(P<0.05)。结论: PEDF抑制SK-MES-1细胞增殖和侵袭,促进细胞凋亡,对肺鳞癌生长和转移发挥对抗效应。提示PEDF可能成为肺鳞癌患者预后判定、抗癌治疗的有效因子。

Effects of PEDF on proliferation,invasion and apoptosis of lung squamous cell carcinoma SK-MES-1 cells

Objective To explore the effects of pigment epithelium derived factor(PEDF) on the proliferation, apoptosis and invasion of lung squamous cell carcinoma SK-MES-1 cells, and to interpret the relationships between PEDF and the carcinogenesis and progression of lung squamous cell carcinoma. Methods The SK-MES-1 cells were primarily cultured.The cells without PEDF were usded as negative control group, the cells in PEDF intervention groups were treated with 180, 360, and 720 nmol·L^-1 PEDF.The inhibitory rates of proliferation of SK-MES-1 cells were observed by MTT assay, the number of SK-MES-1 cells penetrating the membrane in negative control group and 720 nmol·L^-1 PEDF group were determined by cell invasion experiment, the morphology of SK-MES-1 cells was observed by inverted microscope, and the apoptotic rates were detected by flow cytometry. Results The inhibitory rates of proliferation of SK-MES-1 cells in PEDF intervention groups were higer than that in negative control group(P<0.01), and there were statistics differences among the PEDF groups(P<0.05).The cells in negative control group grew well, but the morphological changes of the cells in intervention groups were obvious.The number of the cells penetrating the membrane in 720 nmol·L^-1 PEDF intervention group (31.6±15.8) was lower than that in negative control group (75.4±19.3)(P<0.01).The apoptotic rates of SK-MES-1 cells were increased with the increasing of PEDF doses, and there were significant differences between different doses of PEDF intervention groups and negative control group (P<0.05). Conclusion PEDF inhibits the proliferation or invasion and promotes the apoptosis of SK-MES-1 cells, and play an effect of inhibiting the growth and metastasis of lung squamous cell carcinoma.PEDF may be a potent factor for the prognosis and treatment of lung squamous cell carcinoma.