| HPLC法测定肾石通颗粒中槲皮素含量 |
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| 引用本文： | 肖峰,张敏. HPLC法测定肾石通颗粒中槲皮素含量[J]. 安徽医药, 2009, 13(1): 34-36 |
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| 作者姓名： | 肖峰 张敏 |
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| 作者单位： | 安徽省阜阳市药品检验所,安徽,阜阳,236015;安徽省阜阳市药品检验所,安徽,阜阳,236015 |
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| 摘 要： | 目的建立肾石通颗粒中槲皮素的含量测定方法。方法采用高效液相色谱法KromasilC18色谱柱（250mm×4．6mm，5μm），以甲醇-0．4％磷酸溶液（50：50）为流动相，检测波长：370nm，流速：1．0ml·min^-1，柱温：25℃。供试品溶液制备采用甲醇回流提取1h，聚酰胺柱层析中用70％乙醇提取效果较好。结果槲皮素在5．20～20．80mg·L^-1的浓度范围内峰面积与量呈良好的线性关系，回归方程为：A=3．858×10^5×C＋8．094×10^3（R=0．99998，n=7）；平均加样回收率可达98．9％，（RSD=0．40％，n=9）。结论本法具有专属、可靠、灵敏度高、干扰小等特点，可作为肾石通颗粒中槲皮素的含量测定方法。
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| 关 键 词： | 高效液相色谱 肾石通颗粒 槲皮素 含量测定 |
Determination of guercetin in shengshitong granule by HPLC |
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| XIAO Feng,ZHANG Min. Determination of guercetin in shengshitong granule by HPLC[J]. Anhui Medical and Pharmaceutical Journal, 2009, 13(1): 34-36 |
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| Authors: | XIAO Feng ZHANG Min |
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| Affiliation: | XIAO Feng,ZHANG Min(Fuyang Intitute of Food , Drug Control,Fuyang 236015) |
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| Abstract: | Aim To estalish a method for determination of the content of quercetin in shenshitong granule. Methods HPLC method was used. The sample was separated by Kromasil C18 column （250 mm×4.6 mm,5 μm）. The mobile phase was methanol-0.4% phosphoric acid （50 ： 50）. The detection wavelength was 370 nm. Flow rate was 1.0 ml · min^-1. the column temperature was at 25℃. Results The linear range of quercetin is between 5.20 mg · L^-1 and 20.80 mg · L^-1. The average recovery is 98.9% （RSD = 0.40% ,n = 9）. Conclusions The method is sensitive, rapid and accurate. It is suitable for determining shenshitong grainule. |
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| Keywords: | HPLC shenshitong grainule quercetin determination |
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