高效液相色谱法测定万古霉素的血药浓度

目的建立测定血清万古霉素质量浓度的高效液相色谱法．．方法以HypersilC,s柱（250mm×4．6mill,10μm）为色谱柱,流动相为乙腈一磷酸二氢钾缓冲液（10：90）,流速为0．8mL／min,检测波长为236nm,以10％硫酸锌（ZnSO4）为沉淀血清蛋白,离心后取上清液直接进样测定。结果万古霉素的低、中、高（1．50,30．82,150．45mg／L）3种质量浓度的平均相对回收率分别为99．33％,98．94％和99．70％,日内及日间精密度的RSD均小于5％（n=5）;分析方法的最低检测质量浓度为0．5mg／L;线性范围为2．o～150mg／L,回归方程为C=0．06108A＋0．0003845,r=0．9999。结论该方法灵敏、准确、简单、快速,适用于血清万古霉素血药浓度的监测和药物代谢动力学研究。

Determination of Serum Concentration of Vancomycin by HPLC

Objective To estsblish a HPLC method for the determination of serum mass concentration of vancomycin. Methods Van- comycin was analyzed by the RP-HPLC system with Hypersil Cs column（250 mm ＊4.6 mm,10 μm） and the mobile phase of ace- tonitrile-potassium dihydrogen phosphate （10：90）.The flow rate was 0.8 mL/min and the UV detection wavelength was 236 nm. With 10% ZnSO4 as the precipitation serum albumin, the supernatant after centrifugation was taken for detection by direct sample in- jection. Results The average recovery rate of vancomycin in low,medium and high concentrations （1.50,30.82,150.45 mg/L） were 99.33% ,98.94% and 99.70% ,respectively. The inter-day and intra-day relative standard deviations（RSD） were less than 5% （n =5）. The minimum detectable concentration of serum vancomycin was 0.5 mg/L. The linear range was 2.0- 150 mg/L. The regress equa- tions was C = 0. 061 08 A ＋ 0. 000 384 5, r = 0. 999 9. Conclusion This method shows to be sensitive,accurate, simple, rapid, and suitable for monitoring the serum concentration of vancomycin and its pharmacokinetic study.