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Diagnosis of prosthetic joint infection by beadmill processing of a periprosthetic specimen
Authors:A.-L. Roux  V. Sivadon-Tardy  T. Bauer  A. Lortat-Jacob  J.-L. Herrmann  J.-L. Gaillard  M. Rottman
Affiliation:1. Université de Versailles Saint-Quentin-en-Yvelines, EA3647, Garches;2. Laboratoire de Microbiologie, Hôpital Raymond Poincaré, Assistance Publique – Hôpitaux de Paris (AP-HP), Garches;3. Laboratoire de Microbiologie, Hôpital Ambroise Paré, AP-HP, Boulogne-Billancourt;4. Unité Toulouse Lautrec, Chirurgie Orthopédique et Traumatologie, Hôpital Raymond Poincaré, Assistance Publique – Hôpitaux de Paris (AP-HP), Garches;5. Chirurgie Orthopédique et Traumatologie, Hôpital Ambroise Paré, AP-HP, Boulogne-Billancourt, France
Abstract:
We report a microbiological process for the documentation of prosthetic joint infection (PJI). Intraoperative periprosthetic tissue samples from 92 consecutive patients undergoing revision surgery for PJI were submitted to mechanized beadmill processing: specimens were aseptically collected in polypropylene vials, filled with sterile water and glass beads and submitted to mechanized agitation with a beadmill. The documentation rate of PJI following culture on solid and liquid media was 83.7% and the contamination rate 8.7%. Final documentation was obtained after overnight culture for 51.9% of cases and with 7 days of broth culture for all documented cases.
Keywords:Arthroplasty  beadmill  microbiology  periprosthetic samples  prosthetic joint infection
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