博莱霉素肺纤维化小鼠血管内皮细胞的研究

目的:研究C57BL/6小鼠肺微小血管内皮细胞血栓调节蛋白(TM)与因子Ⅷ相关抗原(vWf)的分布及博莱霉素(BLM)致肺纤维化过程中,血管内皮细胞亚型的转变。方法:采用双重免疫荧光染色及荧光强度定量分析法。结果:①正常C57BL/6小鼠肺泡毛细血管内皮细胞表面显示多数连续性线样TM荧光而vWf较少阳性,肺微小血管内皮细胞呈现vWf阳性。②BLM组小鼠内皮细胞TM荧光明显弱于正常,而vWf荧光显著强于正常水平。结论:①正常C57BL/6小鼠肺泡毛细血管内皮细胞以TM表达为主型,而非vWf表达为主型。②BLM致肺纤维化过程中,肺血管内皮细胞由以TM表达为主型转变为以vWf表达为主型,两抗原可被认为是内皮细胞损伤的标志物。

Study on thrombomodulin and von Willebrand factor in microvasculaturein mice lungs with pulmonary fibrosis induced by bleomycin

AIM:To explore the distribution of thrombomodulin (TM) and von Willebrand factor (vWf) on endothelial cells of lung microvasculature as well as the phenotype change of this cell in the process of pulmonary fibrosis in C57BL/6 mice. METHODS:It was carried out with dual immunofluorescent stain and quantitative analysis of fluorescent intensity of TM and vWf in normal and pulmonary fibrosis model induced by bleomycin (BLM) administration. RESULTS:① The normal lungs showed multiple continuous linear positive staining of TM and seldom positive of vWf on the surface of endothelium of alveolar capillaries. Meanwhile, blood vessels exhibited considerable positive of vWf in endothelial cell in normal C57BL/6 mice. ② The fibrotic lungs revealed a statistically significant diminution of TM expression, and at the same time, an increase of vWf expression when comparing with normal lung sections.CONCLUSION:These results suggest that TM dominant phenotype endothelial cells, rather than vWf dominant phenotype, are the major ones of alveolar capillaries in normal C57BL/6 mice lungs. TM dominant phenotype endothelial cells changed into vWf dominant ones as pulmonary fibrosis develops. Both TM and vWf antigen might be considered as markers of endothelium injury of lung microvasculature.