uPA和MMP-9在乳腺浸润性导管癌中的表达及其相关性

目的探讨尿激酶型纤溶酶原激活剂（uPA）和基质金属蛋白酶（MMP-9）在乳腺癌及肿瘤周围组织中的表达与乳腺癌侵袭、转移的关系。方法应用免疫组化Envision法检测50例乳腺癌及20例癌旁组织中uPA和MMP-9蛋白的表达情况，并分析其相关性及与临床病理特征进行比较。结果乳腺癌组织中uPA的阳性表达率为60％（30／50），MMP-9的阳性表达率为66％（33／50），显著高于癌旁组织（P〈0．01），在乳腺癌组织中uPA、MMP-9蛋白表达之间存在显著正相关（r=0．630，P〈0．01）。uPA、MMP-9表达与淋巴结转移、临床分期显著相关（P〈0．05），与患者的年龄、肿瘤大小无明显相关性（P〈0．05）。结论乳腺癌组织中存在uPA、MMP-9的高表达；uPA蛋白可通过诱导MMP-9蛋白的袁达上调，促进乳腺癌侵袭、转移。

Relationship between the expression of uPA and MMP-9 in invasive ductal carcinoma of breast

Objective To study the expression and correlation of urokinase-type plasminogen activator（uPA） and matrix metaUopmteinase-9 （MMP-9） in invasive ductal carcinoma of breast. Methods Expression of uPA and MMP-9 was detected in 50 cases of breast carcinoma tissue and 20 patients with normal breast tissues adjacent to tumor was examined by immunohistochemi- cal staining. Results The positive expression rates of uPA and MMP-9 in breast carcinoma were 60% ,66% respeetively, show- ing a significant difference between breast carcinoma and normal breast tissues adjacent to tumor（P〈0. 01 ）. The expression of uPA was positively correlated with expression of MMP-9 in breast carcinoma （r = 0. 630, P〈 0. 01 ）. The expression of uPA and MMP-9 was significantly correlated with lymphatic metastasis and surgical-pathologic staging（P〈0. 05） ,but not significantly cor- related with age and tumor size （P〉0.05）. Conclusion The overexpression of uPA and MMP-9 is found in breast carcinoma. To induce uPA overexpression may be one of pathways of MMP-9 that enhances invasion and contributes to the infiltration and metas- tasis in human breast carcinoma.