苯对尾悬吊大鼠血细胞及脂质过氧化的影响

目的:探讨苯吸入染毒对尾吊模拟失重大鼠血液系统、脂质过氧化水平及抗氧化酶的影响.方法:将20只雄性SD大鼠随机分为2组:对照组和染毒组.对照组吸入新鲜空气,染毒组吸入10 mg/m3的苯气体,每天24 h,连续7 d.结果:与对照组相比,染毒组中白细胞(WBC)显著低于对照组(P＜0.05);血红蛋白(HGB)显著低于对照组(P＜0.05);淋巴细胞(LYM)极显著低于对照组(P＜0.01);嗜碱性粒细胞(BAS)显著高于对照组(P＜0.05);T淋巴细胞亚群分类:CD4极显著降低(P＜0.01)、CD8极显著升高(P＜0.01).染毒组中血清超氧化物歧化酶SOD活力极显著低于对照组(P＜0.01);血清谷胱甘肽过氧化酶GSH-PX活力极显著高于对照组(P＜0.01).结论:苯吸入染毒7 d可导致尾吊SD大鼠血液学指标的改变,表现为WBC降低,HGB降低,淋巴细胞(LYM)降低,BAS升高;HGB明显降低,RDW明显升高.脂质过氧化及抗氧化酶指标的改变,表现为SOD活力降低和GSH-PX活力升高.

Effects of Benzene Exposure on Blood System and Lipidperoxidation in Rats Weightlessness Simulated by Tail-suspension

Objective：To study effects of benzene exposure on blood systemand lipid peroxidation and activity of anti-exidase in rats weightlessness simulated by tail-suspension.Methods：Twenty Male SD rats ware divided randomly into two groups,control group with air and benzene group with breathed at the dose of 10 mg/m3 benzene for 7 days.Results：As compared with control group,white blood cell（WBC） decreased significantly（P0.05）,blood hemoglobin（HGB） decreased significantly（P0.05）,blood lymphocyte（LYM） decreased especial significantly（P0.01）.basophil（BAS） increased significantly（P0.05）,blood T-Lymphocytes subgroup typing：CD4 decreased especial significantly（P0.01）,CD8 increased especial significantly（P0.01）.Activity of superoxide dismutase（SOD） decreased especial significantly（P0.01）,activity of glutathione peroxidase（GSH-PX） increased especial significantly（P0.01）.Conclusions：The above results showed that benzene exposure could cause change in white blood cell differential count,blood lymphocyte（LYM） decreased especial significantly;SOD decreased especial significantly,and compensatively increase activity of GSH-PX.