高浓度矽尘接触对大鼠氧化应激的作用研究

目的探讨大鼠高浓度矽尘接触过程中是否存在氧化应激反应。方法选40只SPF级Wistar雄性大鼠随机分为4组,即高剂量染尘组（1 000mg/m^3）、中剂量染尘组（500mg/m^3）、低剂量染尘组（100mg/m^3）和对照组,选用自然动式染尘装置每天染尘2h。染尘49d后处死大鼠,测定肺组织匀浆超氧化物歧化酶（SOD）、总抗氧化能力（T-AOC）活性及丙二醛（MDA）、还原性谷胱甘肽（GSH）含量。结果长时间、高浓度矽尘接触降低大鼠肺组织的SOD（30.25±0.49）U/ml、T-AOC活性（7.93±0.74）kU/L和GSH（2.34±0.96）g/L含量,同时MDA（5.65±0.13）nmol/ml水平升高,与对照组比较,差异有统计学意义（P〈0.01）。结论大鼠高浓度矽尘接触过程早期炎症反应发生可能与机体氧化应急有关。

Study on Effect of High Concentration of Silica Dust Exposure to Oxidative Stress in Rats

Objective To study the effect of oxidative stress in rats exposed to high concentrations of silica dust. Methods A total of 40 male rats were divided into four groups,control（0 mg/kg） ,low （100 mg/m^3） ,moderate （500 mg/m^3） and high （1 000 mg/m^3） dose groups in the test. The rats were treated with silica dust inhaled 2h per day for 49 consecutive days. The lung were collected to observe superoxide dismutase （SOD）, total antioxidant capacity （T-AOC） activity, malondialdehyde （MDA） and glutathione （GSH） content. Results Compared with the control group, SOD（30.25 ± 0.49）U/ml,T-AOC（7.93±0.74）kU/L activity and GSH（2.34±0.96）g/L content in lung tissue homogenates of high dose groups decreased （ P 〈0.05）,and the content of MDA（5.65±0. 13）nmol/ml in lung tissue homogenates of high dose groups increased （ P 〈0.05）. Conclusion The inflammatory response may be related to oxidative stress of body, during the early time of rats exposed to high concentrations of silica dust.