碱性成纤维细胞生长因子受体抗体对人晶状体上皮细胞超微结构的影响

目的:为研究碱性成纤维细胞生长因子(basic fibroblastgrowth factor,bFGF)及其受体抗体对人晶状体上皮细胞(human lens epithelial cells,HLECs)超微结构的影响。方法:采用透射电镜观察正常HLECs及bFGF作用后及受体抗体阻断bFGF在HLECs作用的超微结构变化。结果:正常HLECs胞内较多的线粒体、粗面内质网和游离核糖体及少量微丝结构,核膜完整,染色质均匀。bFGF作用后表现为细胞膜完整,膜局部突起较多,线粒体和游离核糖体明显增多,基质较致密,细胞核附近有较多微丝结构,核膜完整,染色质均匀,核仁增大。bFGF受体抗体阻断作用后表现为细胞突起消失、细胞膜明显破坏,大量线粒体空化,嵴消失,基质减少,呈云雾状退化,内质网明显减少至消失,核膜破坏明显,有的细胞结构不清,趋于死亡。结论:bFGF对HLECs的促增殖作用可被FGF受体抗体所阻断。

Ultrastructure of human lens epithelial cells affected by basic fibroblast growth factor receptor antibodies

AIM:To study ultrastructure of human lens epithelial cells(HLECs)affected by basic fibroblast growth factor(bFGF)and fibroblast growth factor receptor1(FGFR1)antibodies.METHODS:The ultrastructure change was observed in the normal HLECs.The HLECs affected by bFGF and blocked by FGFR1 antibodies using transmission electron microscopy.RESULTS:In normal HLECs,more mitochondrion,rough endoplasmic reticulum,ribosome,and less microfilament were observed.It showed complete nuclear membrane and uniform chromatin.In HLECs affected by bFGF,uniform member and more process in members could be seen.There were evident increasing mitochondrion and ribosome.It showed dense plasma.There were more microfilaments around nuclear.It showed complete nuclear membrane and uniform chromatin.In HLECs blocked by FGFR1 antibodies,cell process disappearing,cell membranes obvious destroying were presented.Number of mitochondrion space existed,ridge disappeared,plasma decreased and showed mist degeneration,endoplasmic reticulum obviously decreased or disappeared,and nucleus membranes obviously destroyed.Some cells were not clear and tended to death.CONCLUSION:The ultrastructure shows that bFGF receptor antibodies are able to black bFGF in stimulating cell proliferation.