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| HCV—specific cytokine induction in monocytes of patients with different oputcomes of hepatitis C | |
| 引用本文： | Woitas RP,Petersen U,Moshage D,Brackmann HH,Matz B,Sauerbruch T,Spengler U. HCV—specific cytokine induction in monocytes of patients with different oputcomes of hepatitis C[J]. World journal of gastroenterology : WJG, 2002, 8(3): 562-566. DOI: 10.3748/wjg.v8.i3.562 |
| 作者姓名： | Woitas RP Petersen U Moshage D Brackmann HH Matz B Sauerbruch T Spengler U |
| 作者单位： | Rainer P.Woitas,Uwe Petersen,Dirk Moshage,Tilman Sauerbruch,Ulrich Spengler(Department of Internal Medicine I, University of Bonn, 53105 Bonn, Germany);Hans H.Brackmann(Institute of Experimental Hematology, University of Bonn, 53105 Bonn, Germany);Bertfried Matz(Institute of Medical Microbiology and Immunology, University of Bonn, 53105 Bonn, Germany) |
| 摘 要： | AIM：Cytokine release by macrophages critically determines the type of immune response to an antigen Therefore.we studied hepatitis C virus (HCV0-Specific induction of interleukins-1β,-10,-12(IL-1β,il-10,IL-12),and tumor necrosis factor-α(TNF-α) in monocytes.METHODS:Intracellular cytokine expression was studied by flow cytometry in 23 patients with chronic hepatitis C,14 anti-HCV seropositives without viremia and 11 controls after stimulation of peripheral blood mononuclear cells with recombinant core,NS3,NS4 NS5a and NS5b proteins .RESULTS:Patients with HCV viremia revealed greater spontaneous exprssion of IL-1β,TNF-α,and IL-10,Furthermore,greater than twofold higher IL-10 epression was induced by the HCV antigens in chronic hepatitis C than in the other two groups (P<0.05) In contrast,neither IL-12 noir TNF-α was induced preferentially.CONCLUSION:In chonic hepatitis C antigen-specific cytokine induction in monocytes is apparently shifted towards predominant IL-10 induction-not counterbalanced by antiviral type 1 cytokines,This may contribute to persistent viral replication. |
| 关 键 词： | HCV－特异细胞动力学 丙型肝炎 预后 |
| 收稿时间： | 2002-03-12 |
HCV-specific cytokine induction in monocytes of patients with different outcomes of hepatitis C |
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| Woitas Rainer P,Petersen Uwe,Moshage Dirk,Brackmann Hans H,Matz Bertfried,Sauerbruch Tilman,Spengler Ulrich. HCV-specific cytokine induction in monocytes of patients with different outcomes of hepatitis C[J]. World journal of gastroenterology : WJG, 2002, 8(3): 562-566. DOI: 10.3748/wjg.v8.i3.562 | |
| Authors: | Woitas Rainer P Petersen Uwe Moshage Dirk Brackmann Hans H Matz Bertfried Sauerbruch Tilman Spengler Ulrich |
| Affiliation: | 1. Department of Internal Medicine I, University of Bonn, 53105 Bonn, Germany 2. Institute of Experimental Hematology, University of Bonn, 53105 Bonn, Germany 3. Institute of Medical Microbiology and Immunology, University of Bonn, 53105 Bonn, Germany |
| Abstract: | AIM: Cytokine release by macrophages critically determines the type of immune response to an antigen. Therefore, we studied hepatitis C virus (HCV)-specific induction of interleukins-1 beta, -10, -12 (IL-1 beta, IL-10, IL-12), and tumor necrosis factor-alpha (TNF-alpha) in monocytes. METHODS: Intracellular cytokine expression was studied by flow cytometry in 23 patients with chronic hepatitis C, 14 anti-HCV seropositives without viremia and 11 controls after stimulation of peripheral blood mononuclear cells with recombinant core, NS3, NS4, NS5a and NS5b proteins. RESULTS: Patients with HCV viremia revealed greater spontaneous expression of IL-1beta, TNF-alpha, and IL-10. Furthermore, greater than twofold higher IL-10 expression was induced by the HCV antigens in chronic hepatitis C than in the other two groups (P<0.05). In contrast, neither IL-12 nor TNF-alpha was induced preferentially. CONCLUSION: In chronic hepatitis C antigen-specific cytokine induction in monocytes is apparently shifted towards predominant IL-10 induction - not counterbalanced by antiviral type 1 cytokines. This may contribute to persistent viral replication. |
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