Ganglioside mapping of a human medulloblastoma xenograft |
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Authors: | J Gottfries J -E Mansson P Fredman C J Wikstrand H S Friedman D D Bigner L Svennerholm |
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Institution: | (1) Department of Psychiatry and Neurochemistry, Gothenburg University, St Jorgens Hospital, Gothenburg, Sweden;(2) Department of Pathology, Duke University Medical Center, Box 3156, 27710 Durham, NC, USA;(3) Department of Pediatrics, Duke University Medical Center, Box 3156, 27710 Durham, NC, USA |
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Abstract: | Summary The ganglioside patterns of medulloblastomas have never been established; in this study we report the ganglioside profile of the human medulloblastoma cell line TE-671 grown as a xenograft in nude mice. Gangliosides were isolated and structurally analyzed by fast atom bombardment mass spectometry following permethylation. Identification of individual gangliosides was also performed by immunostaining of high-performance thin-layer chromatography-separated bands. Total ganglioside levels of 0.20 mol/g of tissue were obtained, consistent with those reported for human glioma cell lines grown as xenografts; predominant monosialogangliosides of TE-671 xenografts were II3--NeuAc-LacCer (GM3) and II3--NeuAc-GgOse3 Cer (GM2) but there were also relatively large proportions of IV3--NeuAc-LcOse4Cer (3-isoLM1), IV3--NeuAc-nLcOse4Cer (3-LM1) and a further ganglioside of the neolactoseries with an extra lactosamine moiety. The only oligosialoganglioside detected was IV3, II3--NeuAc2-GgOse4Cer (GD1a).Abbreviations: The gangliosides have been designated according to Svenerholm 18] GM3
II3--NeuAc-LacCer
- GM2
II3--NeuAc-GgOse3Cer
- GM1
II3--NeuAc-GgOse4Cer
- 3-LM1
IV3--NeuAc-nLcOse4Cer
- 3-isoLMI
IV3--NeuAc-LcOse4Cer
- Fuc-3-isoLMI
IV3--NeuAc, III4-Fuc-LcOse4Cer
- GD1a
IV3, II3--NeuAc2-GgOse4Cer
- FAB-MS
Fast atom bombardment-mass spectometry
- GC-MS
gas chromatography-mass spectometry
Supported by NC1 RO1 CA11898 to Dr. Bigner and B8803X-00627-24B from the Swedish Medical Research Council to Dr. L. Svennerholm |
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Keywords: | Ganglioside Medulloblastoma Xenograft |
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