| 丝素蛋白膜与小鼠胚胎成纤维细胞的体外生物相容性研究 |
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| 引用本文： | 谢军军,熊杰,刘小玲,沈悦娣,尹洪萍,肖红伟,周卸来,唐圣奎.丝素蛋白膜与小鼠胚胎成纤维细胞的体外生物相容性研究[J].杭州医学高等专科学校学报,2011(3):169-171,174,F0003. |
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| 作者姓名： | 谢军军 熊杰 刘小玲 沈悦娣 尹洪萍 肖红伟 周卸来 唐圣奎 |
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| 作者单位： | 杭州师范大学附属医院;浙江理工大学先进纺织材料与制备技术教育部重点实验室; |
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| 基金项目： | 973计划前期研究专项课题(2008CB617506); 杭州市卫生科技计划项目(2009A013); 杭州市科技发展计划项目(20090833B24) |
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| 摘 要： | 目的探讨不同结构的丝素蛋白支架材料对小鼠胚胎成纤维细胞（NIH-3T3）生长繁殖性能的影响。方法将NIH-3T3接种在丝素蛋白纳米纤维膜和浇铸膜上进行体外培养，并设空白对照组。通过MTT法、HE染色、扫描电镜来分析不同结构丝素蛋白膜的生物相容性。结果增殖培养4天时实验组的OD值与对照组相比，有显著性差异（P〈0．05）；增殖培养7天时实验组与空白组相比无显著性差异（P〉0．05），而对照组与空白组相比有显著性差异（P〈0．05）。HE染色、扫描电镜显示NIH-3T3细胞在纳米纤维膜上比在浇铸膜上有更好的生长和连接状态。结论与丝素蛋白浇铸膜相比，NIH-3T3细胞在纳米纤维膜上表现出更好的增殖能力，无明显细胞毒性表现；丝素蛋白纳米纤维膜具有良好的生物相容性，是皮肤组织工程的良好载体。
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| 关 键 词： | 丝素蛋白 纳米纤维膜 小鼠胚胎成纤维细胞（NIH-3T3） 生物相容性 |
A Study on in Vitro Biocompatibility between Silk Fibroin Membranes and Mouse Embryo Fibroblasts |
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| XIE Jun-jun,XIONG Jie,LIU Xiao-ling,SHEN Yue-di,YIN Hong-ping,XIAO Hong-wei,ZHOU Xie-lai,TANG Sheng-kui.A Study on in Vitro Biocompatibility between Silk Fibroin Membranes and Mouse Embryo Fibroblasts[J].Journal of Hangzhou Medical College,2011(3):169-171,174,F0003. |
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| Authors: | XIE Jun-jun XIONG Jie LIU Xiao-ling SHEN Yue-di YIN Hong-ping XIAO Hong-wei ZHOU Xie-lai TANG Sheng-kui |
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| Institution: | XIE Jun-jun1,XIONG Jie2,LIU Xiao-ling1,SHEN Yue-di1,YIN Hong-ping1,XIAO Hong-wei2,ZHOU Xie-lai,TANG Sheng-kui2(1.Clinical Medical College,Hangzhou Normal University,Hangzhou,Zhejiang 310036,China,2.Key laboratory of Advanced Textile Materials and Manufacturing Technology,Ministry of Education,Zhejiang Sci-Tech University,Zhejiang 310018,China) |
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| Abstract: | Objective To explore the functions of different silk fibroin membranes to the growth and the proliferation of mouse embryo fibroblasts(NIH-3T3). Methods NIH-3T3 cells were cultured on silk fibroin nanofibrous and casting membranes.The biocompatibility of silk fibroin membranes with different structures were studied by MTT,HE staining,scanning electron microscope methods Results There was a significant difference(P<0.05) between the experimental group and the control when cellshave grown for 4 days.NIH-3T3 c... |
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| Keywords: | Silk fibroin nanofibrous membrane mouse embryo fibroblasts(NIH-3T3) biocompatibility |
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