| Pharmacological Investigation of Voltage-dependent Ca^2＋ Channels in Human Ejaculatory Sperm in vitro |
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| 引用本文： | 李路,刘继红,李家贵,叶章群.Pharmacological Investigation of Voltage-dependent Ca^2＋ Channels in Human Ejaculatory Sperm in vitro[J].华中科技大学学报(医学英德文版),2006,26(5):607-609. |
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| 作者姓名： | 李路 刘继红 李家贵 叶章群 |
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| 作者单位： | Department of Urology Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430030，China，Department of Urology，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430030，China，Department of Urology，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430030，China，Department of Urology，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430030，China |
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| 摘 要： | The types of the voltage-dependent calcium channels (VDCCs) in human ejaculatory sperm and the effects of calcium channel blocker (CCB) on human sperm motility parameters in vitro were investigated. The human sperm motility parameters in vitro in response to the pharmacological agents nifedipine (NIF, inhibitor of L-type VDCC) andω-conotoxin (GVIA, inhibitor of N-type VDCC) were compared and analyzed statistically. The results showed that NIF (1, 5, 10μmol/L) could not only significantly affect human sperm's shape but also spermatozoa motility after incubated at least 10 min in vitro (P<0.001). GVIA (0.1, 0.5 and 1μmol/L) could just only significantly affect human sperm's progressive motility (a % b %) after incubated for 20 min in vitro (P<0.01), but they both could not significantly affect spermic abnormality rate. It is suggested that L-type VDCC, non L-type VDCCs and isoform of L-type VDCC exist in the cell membrane of human sperm solely or together, and they participate in the spermic physiological processes especially the spermic motility.
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| 关 键 词： | 药理学 钙离子 实验 研究 |
| 收稿时间： | 2005-12-12 |
Pharmacological investigation of voltage-dependent Ca2+ channels in human ejaculatory sperm in vitro |
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| Lu Li,Jihong Liu,Jiagui Li,Zhangqun Ye.Pharmacological investigation of voltage-dependent Ca2+ channels in human ejaculatory sperm in vitro[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2006,26(5):607-609. |
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| Authors: | Lu Li Jihong Liu Jiagui Li Zhangqun Ye |
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| Institution: | Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030,China |
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| Abstract: | Summary The types of the voltage-dependent calcium channels (VDCCs) in human ejaculatory sperm and the effects of calcium channel
blocker (CCB) on human sperm motility parameters in vitro were investigated. The human sperm motility parameters in vitro
in response to the pharmacological agents nifedipine (NIF, inhibitor of L-type VDCC) and θ-contoxin (GVIA, inhibitor of N-type
VDCC) were compared and analyzed statistically. The results showed that NIF (1, 5, 10 μmol/L) could not only significantly
affect human sperm’s shape but also spermatozoa motility after incubated at least 10 min in vitro (P<0.001). GVIA (0.1, 0.5 and 1 μmol/L) could just only significantly affect human sperm’s progressive motility (a %+b %) after
incubated for 20 min in vitro (P<0.01), but they both could not significantly affect spermic abnormality rate. It is suggested that L-type VDCC, non L-type
VDCCs and isoform of L-type VDCC exist in the cell membrane of human sperm solely or together, and they participate in the
spermic physiological processes especially the spermic motility. |
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| Keywords: | voltage-dependent calcium channels calcium channel blocker human sperm motility in vitro |
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