245例人类免疫缺陷病毒感染者唾液中人类疱疹病毒1～4型的检测情况分析

目的探讨人类免疫缺陷病毒（HIV）感染者唾液中人类疱疹病毒（HHV）1～4型即单纯疱疹病毒1型（HSV-1）、单纯疱疹病毒2型（HSV-2）、水痘-带状疱疹病毒（VZV）和EB病毒（EBV）的分布情况。方法利用聚合酶链反应（PCR）方法对HIV感染者和健康者非刺激性全唾液中HSV-1、HSV-2、VZV和EBV DNA的存在情况进行检测，并采用SPSS 18.0软件建立数据库并进行相关指标的统计分析。结果在245例HIV阳性患者唾液中，HSV-1、HSV-2、VZV和EBV检出率分别为29.0%、3.3%、4.1%和82.0%；30例对照组唾液中上述4种病毒检出率分别为13.3%、0、0和36.7%。两组总体检出率比较差异有统计学意义（P<0.01）。高效抗逆转录病毒治疗（HAART）使用后HIV感染者唾液中4种病毒的检出率与未使用HAART的患者差异均无统计学意义（P>0.05）。结论云南地区HIV感染者HHV有较高的流行率，以EBV最为常见，其次为HSV-1，VZV和HSV-2少见，还存在多种疱疹病毒联合感染。

Incidence of human herpes virus 1-4 type in saliva of 245 human immunodeficiency virus-infected patients

Objective To determine the incidence of human herpes virus（HHV） 1-4 type including herpes simplex virus type-1（HSV-1）, herpes simplex virus type-2（HSV-2）, varicella-zoster virus（VZV）, Epstein-Barr virus（EBV）in the saliva of human immunodeficiency virus（HIV）-infected patients. Methods The incidence of salivary HSV-1, HSV-2, VZV and EBV from 245 HIV-seropositive individuals and control group was used to investigate by polymerase chain reaction（PCR） or nested PCR. The data was analyzed by SPSS 18.0 statistical software. Results In the 245 HIV-seropositive individuals, the detection rates of HSV-1, HSV-2, VZV, EBV were 29.0%, 3.3%, 4.1%, 82.0%. In the control group, the detection rates of HSV-1, HSV-2, VZV, EBV were 13.3%, 0, 0, 36.7%. Four HHVs were significantly
more prevalent in the salivas of HIV-seropositive persons than those in the control group（P<0.01）. The detection rates of HSV-1, HSV-2, VZV and EBV DNA were no difference between the HIV-positive group with highly active antiretroviral therapy（HAART） and HIV-positive group without HAART（P>0.05）. Conclusion There is a high prevalence of HHV infection in HIV-infected people in Yunnan. The most common virus are EBV, followed by HSV-1, but VZV and HSV-2 are rarely detected. HHV co-infection is also observed.