早期绒毛和足月分娩胎盘组织中雌二醇水平测定及其意义

目的：通过检测自然妊娠早期孕妇外周血及绒毛组织、足月分娩时孕妇外周血、胎盘组织以及脐动脉血中雌二醇（E2）水平，并体外培养滋养层细胞系BeWo细胞，探讨E2的作用。方法：本研究纳入20例非意愿妊娠行妊娠早期流产者、20例足月妊娠行剖宫产者，采用化学发光免疫分析（CLIA）检测妊娠早期流产绒毛组织、孕妇外周血以及足月分娩时孕妇外周血、脐动脉血和胎盘组织中E2水平。单层培养BeWo细胞，用0 mol/L、5×10-7 mol/L、5×10-8 mol/L、5×10-9 mol/L浓度的E2各处理1，3，6，12 h后，用Millicell ESR-2测定其跨膜电阻（TEER），观察BeWo细胞间紧密连接的完整性。结果：妊娠早期的孕妇血清E2浓度[（3.44±1.30） nmol/L]低于足月妊娠孕妇[（509.78±197.82）nmol/L]，差异有统计学意义（t=10.2，P＜0.001）；而绒毛组织中E2浓度[（30.82±13.91）pmol/mg]显著高于足月胎盘组织中E2浓度[（17.21±5.37）pmol/mg]，差异有统计学意义（t=4.1，P＜0.001)）。用5×10-7 mol/L的E2处理BeWo细胞12 h后，TEER下降（P＜0.05）；5×10-8 mol/L和5×10-9 mol/L E2处理对BeWo细胞TEER无明显影响（P＞0.05）。结论：妊娠早期绒毛组织中含有高浓度的E2，足月分娩时胎盘组织中E2相对较低；在体外实验中，不同浓度雌激素可能影响胎盘滋养细胞连接功能的维持，5×10-7 mol/L可作为E2处理BeWo细胞的有效浓度。

Placental and Villous Concentrations of Estradiol in the Pregnancy and Its Significance

Objective:To test estradiol (E2) levels in the maternal peripheral blood, villi and placenta in the first trimester and term parturition, as well as the estradiol level of umbilical artery blood in term delivery. Culture trophoblast cell line BeWo in vitro to investigate the role of E2. Methods:This study included 20 unwilling pregnant women who want to have early artificial abortion and 20 pregnant women of full term cesarean delivery. The levels of E2 in the tissues of early abortion, peripheral blood of pregnant women and umbilical artery blood, placenta tissue of the women during labor were measured by chemiluminescence immunoassay(CLIA). After BeWo cells in monolayer culture, those cells were treated with E2 of 0 mol/L, 5×10-7 mol/L, 5×10-8 mol/L and 5×10-9 mol/L for 1, 3, 6 and 12 h, and then transepithelial resistance (TEER) was measured by millicell ESR-2 to observe the integrity of the tight junctions between BeWo cells. Results:E2 were lower in peripheral plasma from early pregnancy [(3.44±1.30)nmol/L] compared with that from third trimesters [(509.78±197.82 ) nmol/L](t=10.2,P<0.001). However, E2 were higher in tissue obtained from villus[(30.82±13.91) pmol/mg] than that from third trimesters placenta[(17.21±5.37)pmol/mg](t=4.1, P<0.001). A decrease of TEER was observed after the treatmeat of E2 at a concentration of 5×10-7 mol/L for 12 hours (P<0.05). The other two concentrations, 5×10-8 mol/L and 5×10-9 mol/L, had no significant effect on the TEER of BeWo cells at all time points(P>0.05). Conclusions:The concentrations of E2 in villus obtained from first trimester is higher;E2 in full-term birth placenta tissues is relatively lower; In vitro, different concentrations of E2 may affect the maintenance of junction between trophoblast cells, 5×10-7 mol/L can be used as the effective concentrations of estradiol to treat with BeWo cells.