Estimation of paracrine signaling using quantitative RT-PCR from multiple patchy lesion samples |
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| Authors: | Shuang Jiao Haruo Hanawa Tomoyasu Suzuki Ying Hua Hu Kaori Yoshida Minako Ogura Yukako Ohno Masahiro Ito Takeshi Kashimura Hiroaki Obata Makoto Kodama |
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| Affiliation: | Division of Cardiology, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan |
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| Abstract: | We investigated whether correlations between mRNA levels of cytokines versus other proteins from patchy lesion could estimate cytokine paracrine signaling in vivo. Experiments with rat experimental autoimmune myocarditis (EAM), a patchy myocarditis model, indicated IL-1 and other protein levels were correlated, indicating paracrine signaling pathways in vivo. |
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