| 乙型肝炎核酸疫苗肌注小鼠后质粒的存续及局部组织内C-myc mRNA的检测 |
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| 引用本文： | 秦山,赵连三,唐红. 乙型肝炎核酸疫苗肌注小鼠后质粒的存续及局部组织内C-myc mRNA的检测[J]. 中华肝脏病杂志, 1999, 7(1): 6-7 |
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| 作者姓名： | 秦山 赵连三 唐红 |
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| 作者单位： | 华西医科大学附属第一医院病毒性肝炎研究室!成都６１００４１ |
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| 基金项目： | 国家自然科学基金!39670670,四川省科委九五攻关重大项目,四川省计委科研基金 |
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| 摘 要： | 研究乙型肝炎核酸疫苗质粒ＮＶ－ＨＢ／ｓ接种小鼠后小鼠产生体液免疫应答的规律,质粒在注射局部的存续规律及对癌基因Ｃ－ｍｙｃ的可能影响。方法 ＥＬＩＳＡ法检测小鼠外周血中的抗ＨＢｓ;分别用原位杂交和Ｓｏｕｔｈｅｒｎ吸印杂交检测小鼠肌组织标本中的疫苗质粒;并以原位杂交检测肌组织中的Ｃ－ｍｙｃ ｍＲＮＡ。结果 小鼠在接种ＮＶ－ＨＢ／ｓ后１月均已产生抗原ＨＢｓ,肌组织中ＮＶ－ＨＢ／ｓＤＮＡ的原位杂交阴性,仅
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| 关 键 词： | 乙型肝炎 质粒 疫苗 核酸 C－myc基因 |
Detection of NV-HB/s DNA and c-myc mRNA in mice inoculated with hepatitis B nucleic acid vaccine NV-HB/s |
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| QIN Shan, ZHAO Liansan, TANG Hong,et al.. Detection of NV-HB/s DNA and c-myc mRNA in mice inoculated with hepatitis B nucleic acid vaccine NV-HB/s[J]. Chinese journal of hepatology, 1999, 7(1): 6-7 |
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| Authors: | QIN Shan ZHAO Liansan TANG Hong et al. |
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| Affiliation: | Viral Hepatitis Research Unit, West China University of Medical Sciences, Chengdu. |
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| Abstract: | Objective To explore the persistence period of plasmid in mice muscles injected withNV-HB/s and the possible influence on oncogen C-myc. Methods Anti-HBs was detected by ELISA,NV-HB/s was detected by In-situ and Southern-blot hybridization separately, C-myc mRNA wasdetected by In-situ hybridization. Results All mice injected with NV-HB/s were positive of anti-HBsafter I month of immunization, NV-HB/s was negative when detected by In-situ hybridization, BySouthern-blot hybridization, the positive rates of NV-HB/s were decreasing from 100% in 2 months to25% after 6 months of inoculation. Conclusions NV-HB/s would be disintegrated little by little afterinjected into mouse muscles; no C-myc activation was observed in 6 months after NV-HB/s inoculation. |
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| Keywords: | Hepatitis B Plasmid Vaccine Nucleic acids C-myc gene |
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