纤维蛋白胶粘合修复周围神经损伤的实验研究

目的 比较纤维蛋白胶粘合法与神经外膜缝合法修复周围神经损伤的效果。方法 将雄性Wistar大鼠20只随机分为两组，即粘合组和缝合组，每组10只。以大鼠左侧坐骨神经为修复神经模型进行实验。术后8周，各组取8只大鼠进行电生理、组织学检查，每组其余2只取标本做透射电镜观察。结果 术后8周粘合组神经纤维较缝合组平直，粘合组与缝合组比较，运动神经潜伏期延迟比、吻合口神经纤维通过比、有髓纤维截面积恢复比、肌湿重恢复比差别具有显著性意义(t值分别为2．32、2．43、2．39、2．36，P值分别为0．032、0．029、0．030、0．031)。结论 应用纤维蛋白胶粘合修复周围神经损伤是一种较为有效的神经接合方法。

An experimental study on injured peripheral nerves repaired by adhesion of fibrin glue

Objective To compare the effects of fibrin glue adhesion or epineurium suture on repairing injured peripheral nerves. Methods 20 male rats were randomly divided into two groups: group fibrin glue adhesion (n=10) and group suture (n=10). Left sciatic nerves of rats were made repaired nerves models. The nerves of 8 rats in each group underwent electrophysiological and histological examination at the 8th week postoperatively. The nerve specimens of other 2 rats in each group were examined under transmission electron microscope. Results Nerve fibers alignment in group adhesion was more regular than that of group suture at the 8th week after operation. There were significant differences in delayed ratio of latency of motor nerve, nerve fibers cross ratio, recovery ratio of sectional area of myelinated fibers and recovery ratio of muscle wet weight between the two groups( t =2 32, P =0 032; t =2 43, P =0 029; t =2 39, P =0 030; t =2 36, P =0 031). Conclusions Adhesion of fibrin glue for the repair of injured peripheral nerves is an effective method.