新疆汉族和维吾尔族妇女子宫内膜异位症中Bcl-2、Bax、survivin的表达及意义

目的：探讨凋亡调节基因Bcl-2、Bax、survivin在汉族和维吾尔族（维族）妇女子宫内膜异位症（EMs）中的表达及意义。方法：44例EMs患者，汉族22例，维族22例；30例非EMs及非内膜病变者作为对照组，汉族15例，维族15例，应用免疫组化法检测EMs患者和对照组子宫内膜组织不同族别、不同时期（增生期、分泌期）及在位内膜、异位内膜中Bcl-2、Bax的表达，同时采用原位杂交法检测survivin的表达。结果：Bcl-2、Bax、survivin主要定位于子宫内膜的腺上皮。（1）维、汉族对照组子宫内膜增生期Bcl-2的表达高于分泌期（P〈0.05），子宫内膜增生期、分泌期survivin都弱表达，差异无统计学意义（P〉0．05）。EMs患者异位、在位内膜中Bcl-2和survivin的表达较对照组明显增强，差异有统计学意义（P〈0．05）。（2）维、汉族对照组子宫内膜增生期和分泌期Bax都强表达，差异无统计学意义（P〉0．05）。EMs患者在位及异位内膜中Bax表达均比对照组减弱，差异有统计学意义（P〈0．05）。结论：EMs中凋亡调节基因的表达与无EMs者不同，促凋亡基因表达减弱，抑制凋亡基因表达增强，有利于异位内膜的种植生存和发展。维族和汉族EMs凋亡基因Bcl-2、Bax、survivin表达无差异。

The expression and significance of Bcl-2, Bax and survivin in Han and Uigur women with endometriosis

Objectives: To evaluate and compare Bcl-2,Bax,survivin gene experession in Han and Uigur women with and without endometriosis(EM) to explore possibility for the pathogenesis of endometriosis.Methods: Bcl-2 and Bax expressions were assessed with immunohistochemical techniques.In situ hybridization techniques was used to examine the survivin mRNA expression,and were examined in the eutopic and ectopic endometrium of patients with endometriosis and eutopic endometrium of without endometriosis.There were 37 Uigurs(15 controls and 22 patients) and 37 Hans(15 controls and 22 patients).(Results:) Bcl-2,Bax and survivin immuneoreactivity were found predominately in the glandular epithelial cells,Bcl-2 and survivin expression in eutopic and ectopic endometrium was significantly increased compared with the healthy controls,and Bax expression in eutopic and ectopic endometrium was significantly decreased compared with the healthy controls(P<0.05).An increased expression of Bcl-2 protein was found in proliferative eutopic endometrium from healthy controls.Bax expression was increased both proliferative and secretory endometrium in healthy controls.There was no significant difference in the expression level of survivin between proliferative and secretory phase in healthy controls(P>0.05).Conclusions: Bcl-2 and survivin expression with EMs was significantly increased compared with no EMs and Bax expression with EMs was significantly decreased compared with no EMs.The results indicate that the expression of apoptotic genes were different in eutopic and ectopic endometrium from patients with endometriosis and without endometriosis.The expression of promoting apoptotic genes decreased and restraning apoptotic genes increased,which could bear implications for the growth and survival of ectopic endometrial tissue.No statistically significant differentce was found in the expression of Bcl-2,Bax and survivin between Hans and Uigurs.