非小细胞肺癌外周血分子微转移研究

目的:研究非小细胞肺癌(NSCLC)患者外周血癌胚抗原(CEA)、CK19、Lunx mRNA表达对于肿瘤微转移的价值.方法:采用RT-PCR技术,对46例NSCLC患者(其中肺癌Ⅰ期组6例,Ⅱ期组9例,Ⅲ期组14例,Ⅳ期组17例))和同期18例良性肺部疾病(对照组)患者外周血CEA、CK19、Lunx mRNA进行检...

Study on circulating molecular marker of micrometastasis in non-small cell lung cancer

Objective：To explore the diagnostic value of carcinoembryonic antigen（CEA）,CK19,Lunx mRNA in peripheral blood of patients for the micrometastasis in non-small cell lung cancer（NSCLC）.Methods： RT-PCR was used to detect the expression of CEA,CK19,and Lunx mRNA in peripheral blood samples from 46 patients with NSCLC and 18 patients with benign pulmonary lesion.Results： The positive rates of Lunx,CEA and CK19 mRNA were 52.2%,43.5% and 41.3% respectively in peripheral blood of patients with NSCLC.The positive rates of Lunx,CEA and CK19 mRNA in stage Ⅰ were 16.6%（1 /6）,16.6%（1 /6） and 0%（0/6） respectively,and they had no significant differences with control（P0.05）.The positive rates of Lunx mRNA in stageⅡ,stage Ⅲ,and stage Ⅳ patients were 44.4%（4/9）,50.0%（7/14）,and 70.6%（12/17） respectively.The positive rates of CEA mRNA were 33.3%（3/9）,50.0%（7/14） and 52.9%（9 /17） respectively.The positive rates of CK19 mRNA were 22.2%（2/9）,42.9%（6/14） and 64.7%（11/17） respectively.The positive rates of Lunx mRNA in stage Ⅱ,CEA mRNA and Lunx mRNA in stage Ⅲ,and Lunx,CEA,CK19 mRNA in stage Ⅳ had significant differences with control（P0.05-P0.01）.Conclusions：The expressions of CEA,CK19 and Lunx mRNA in peripheral blood can be used as diagnostic biomarkers of micrometastasis in patients with NSCLC.