叶绿素光敏剂选择性损伤鸡冠血管参数选择

通过观察光动力治疗后鲜红斑痣动物模型鸡冠的形态学变化,选择光敏剂损伤鸡冠微血管的最佳参数。方法：１０２只动物随机分成６组６组,３组对照组（空白对照、单纯照光组、单纯光敏剂组）;３组实验组,分别给予不同的光敏剂浓度（５、７．５、１０、１２．５、１５ｍｇ／ｋｇ体重）,功能密度（５０、１００、１５０、２００ｍＷ／ｃｍ＾２＿,能量密度（１５、３０、４５、６０、１２０、１３５、１８０Ｊ／ｃｍ＾２）及给药至照

Optimal Treatment Parameters for Damage of Chicken Comb Vessels Using Chlorophyll Derivative V in Photodynamic Therapy

OBJECTIVE: To define the optimal treatment parameters of chlorophyll derivative IV (CPD4) in photodynamic therapy(PDT) of port wine stains(PWS) by observing the morphological changes of chicken combs. METHODS: 102 animals were divided into 6 groups at random, including 3 control groups(given no treatment, given light without photosensitizer or given photosensitizer without light) and 3 experimental groups treated with different CPD4 dosages(5, 7.5, 10, 12.5, 15 mg/kg body weight), power density(50, 100, 150, 200 mW/cm2), energy density (15, 30, 45, 60, 120, 135, 180 J/cm2) and different intervals between CPD4 injection and irradiation (immediately, 5, 10, 20, 30, 60, 120, 180, 240 minutes). The main emitting peak of light source was at 650 nm, and CPD4 was injected intravenously, then the appearance of chicken comb was recorded everyday. The prepared sections were stained with HE. The histological changes of chicken comb were observed by light microscope. RESULTS: The control animals showed no significant comb changes, while the PDT could cause blanching of the chicken comb in the experimental group. The histologic changes included damage of erythrocytes and endothelial cells, vascular rupture, hemorrhage or disappearance, denaturation of perivascular collagen fiber and local necrosis of chicken combs. The treatment parameters which caused the most obvious blanching of the chicken comb were 7.5-10 mg/kg body weight of CPD4 dosage, 100-150 mW/cm2 of power density, 60-90 J/cm2 of energy density, and the intervals between CPD4 injection and irradiation were 0-20 minutes. CONCLUSION: CPD4 in PDT can cause selective damage of dermal capillaries in chicken comb and blanching of the chicken comb with optimal treatment parameters. The results will offer the basis for further investigation of clinic treatment parameters.