盆腔粘连与沙眼衣原体和解脲支原体感染的关系

目的　探讨沙眼衣原体 (chlamydiatrachomatis,CT)和解脲支原体 (ureaplasmaurealyticum ,UU)感染与盆腔粘连的关系及其发病机制 ,并试图找到预防和降低盆腔粘连发生的方法和途径。方法　分别采用聚合酶链反应 (PCR)技术、金标免疫斑点法 ,将 5 0例妇科既往无盆腔手术史 ,行腹腔镜和开腹手术中有盆腔粘连患者作为盆腔粘连组 (观察组 ) ,4 0例同期妇科腹腔镜和开腹手术中无盆腔粘连患者为对照组。测定宫颈分泌物和手术标本中的CTDNA、UUDNA ,以及两组患者血清中抗CT和UU抗体。结果　观察组宫颈分泌物CT和UUDNA阳性率分别为 32 %和 4 0 % ;血清CT和UU抗体阳性率分别为 34%和 4 8% ;手术标本CT和UUDNA阳性率分别为 2 6 %和 30 %。对照组宫颈分泌物CT和UUDNA阳性率分别为 7 5 %和 1% ;血清CT和UU抗体阳性率分别为 5 %和 10 2 5 % ;手术标本CT和UUDNA阳性率分别为 2 5 %和 5 %。两组比较差异有显著性 (P <0 0 1)。结论　沙眼衣原体、解脲支原体感染与盆腔粘连有着密切关系。

Relationship between chlamydia trachomatis or ureaplasma urealyticum infection and pelvic adhesion

Objective To investigate the relationship between Chlamydia trachomatis (CT) or Ureaplasma urealyticum (UU) infection and pelvic adhesion. To explore the mechanism of pelvic adhesion and the possible approach to reduce the incidence of pelvic adhesion. Methods 50 women with pelvic adhesion in laparoscopic of abdominal operation (study group) and 40 women without pelvic adhesion in the same period (control group) were recruited in this study. Cervical discharge samples and operational samples were collected and CT DNA and UU DNA were detected by polymerize chain reaction (PCR). Serum samples were used to detect CT antibody and UU antibody by gold-labeled immunospot. Results CT DNA and UU DNA were detected in cervical discharge at rates of 32% and 40% respectively in the study group, and 7.5% and 1% respectively in the control group. The positive rate of CT-Ab and UU-Ab in the study group were 34% and 48% respectively, 5% and 10.25% in the control group respectively. CT DNA and UU DNA were detected in operational samples at rates of 26% and 15% respectively, and 2.5% and 5% in controls. All of the differences between the study group and control were statistically significant (P<0.01). Conclusions Chlamydia trachomatis (CT) and Ureaplasma urealyticum (UU) infections were correlated with pelvic adhesion.