红背丝绸提取物和单体CA-1201对内皮素1效应的拮抗作用

目的：观察红背丝绸２种提取物和单体（ＣＡ１２０１）对内皮素１（ｅｎｄｏｔｈｅｌｉｎ１，ＥＴ１）生物效应的拮抗作用。方法：用红背丝绸３种提取物对ＥＴ１致小鼠急性死亡、血管收缩和升压作用的拮抗，探索用中草药拮抗ＥＴ的生物效应。结果：３种提取物对ＥＴ１致小鼠急性死亡有明显的保护作用，使小鼠的死亡率下降；并可拮抗ＥＴ１致胸主动脉的缩血管效应，醇提物和乙酸乙酯提取物（１０－７母液）拮抗ＥＴ缩血管效应分别为５７．２７％和５８．６１％；ＣＡ１２０１１０－８ｍｏｌ·Ｌ－１和１０－９ｍｏｌ·Ｌ－１亦能拮抗ＥＴ１的缩血管效应；醇提物和乙酸乙酯提取物ｉｇ及ＣＡ１２０１ｉｖ后能拮抗ＥＴ１的升压作用，拮抗ＥＴ１作用均呈现明显的剂量依赖性。结论：红背丝绸２种提取物和ＣＡ１２０１可拮抗ＥＴ１的生物学效应

Antagonism effects of both extracts of Chinese herb Cissus assamica and monomer CA-1201 on endothelin 1 responses

OBJECTIVE: To observe the antagonism effects of both alcohol and ethyl acetate extracts, monomer CA 1201 three components from Chinese herb Cissus assamica on endothelin 1 (ET 1) biological responses in vivo and in vitro. METHODS: We used both extracts and monomer CA 1201 to antagonize mouse acute death and vasoconstriction and blood pressure elevation induced by ET 1. RESULTS: These components could protect the acute death induced by ET 1 with reduce of death rate; and antagonize the vasoconstriction induced by ET 1 on isolated arotic ring of rats; both alcohol and ethyl acetate extracts through oral administration, CA 1201 iv route could blunt effects of elevation of blood pressure induced by ET 1, the effects of these components were in dose dependent manner. CONCLUSION: Both extracts and monomer CA 1201 from Chinese herb Cissus assamica can antagonize ET 1 biological effects.