| 甲基苯丙胺对大鼠纹状体小胶质细胞及NOS的影响 |
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| 引用本文： | 李艳红,王慧君,乔东访.甲基苯丙胺对大鼠纹状体小胶质细胞及NOS的影响[J].南方医科大学学报,2008,28(10):1789-1791. |
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| 作者姓名： | 李艳红 王慧君 乔东访 |
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| 作者单位： | 南方医科大学法医学研究所,广东,广州,510515 |
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| 摘 要： | 目的 观察甲基苯丙胺(METH)对大鼠纹状体内小胶质细胞的影响以及一氧化氮合酶(NOS)、诱导型一氧化氮合酶(iNOS)和结构性一氧化氮合酶(cNOS)的变化以及相互间的关系.方法 建立METH给药模型,实验组给予METH,对照组给予等体积生理盐水,采用OX-42免疫组化观察小胶质细胞的变化情况,采用酶化学方法测量纹状体内的NOS、iNOS和cNOS的活性改变.结果 与对照组相比,实验组纹状体区小胶质被细胞激活,数量显著增加(P<0.01),呈现为"灌木丛"(bushy)甚至"阿米巴样"(amoeboid),同时NOS、iNOS和cNOS的活性均显著升高(P<0.01).结论 小胶质细胞被激活,NOS活性升高,可能是METH引起中枢神经损伤的重要机制.
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| 关 键 词： | 甲基苯丙胺 小胶质细胞 诱导型一氧化氮合酶 |
Effect of methamphetamine on the microglial cells and activity of nitric oxide synthases in rat striatum |
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| LI Yan-hong,WANG Hui-jun,QIAO Dong-fang.Effect of methamphetamine on the microglial cells and activity of nitric oxide synthases in rat striatum[J].Journal of Southern Medical University,2008,28(10):1789-1791. |
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| Authors: | LI Yan-hong WANG Hui-jun QIAO Dong-fang |
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| Institution: | LI Yan-hong,WANG Hui-jun,QIAO Dong-fang Institute of Forensic Medicine,Southern Medical University,Guangzhou 510515,China |
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| Abstract: | Objective To study the changes in the microglial cells and the activity of nitric oxide synthase(NOS), inducible nitric oxide synthase(iNOS) and constitutive nitric oxide synthase(cNOS) in the striatum of rats with methamphetamine(METH) treatment.Methods The rats were randomly divided into two groups for injections with METH or saline.Specific antibody against OX-42 was used to detect the changes in the morphology and the number of microglia, and the activities of NOS, iNOS and cNOS were compared between th... |
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| Keywords: | methamphetamine micraglia inducible nitric oxide synthase |
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