HGV/GBV-C感染在肝细胞损伤中的作用探讨

目的 研究单一ＨＧＶ／ＧＢＶ－Ｃ感染者肝组织中该病毒核酸定位及相关抗原表达，探讨ＨＣＶ／ＧＢＶ－Ｃ感染在肝细胞损伤中的作用。方法 １２例单一ＨＧＶ／ＧＢＶ－Ｃ感染者经肝穿获得取肝组织常规病理诊断，采用地高辛标记探针原位杂交法检测病毒ＲＮＡ，并用免疫组织化学法检测病毒相关抗原表达。结果 病理诊断急性肝炎８例，慢性肝炎４例。ＨＧＶ／ＧＢＶ－ＣＮＳ５抗原检出阳性率为６６．６７％（８／１２），阳性信号主要

Study of the mechanism of liver cells injury after HGV/GBV-C infection

Objective To study the exppression of hepatitis G virus or GB virus C (HGV/GBV C) RNA and virus related antigens in the liver of patients with HGV/GBV C infection, and the mechanisms of liver cells injury induced by HGV/GBV C. Methods The expression of HGV/GBV C RNA in the liver tissues were detected with digoxigenin labeled probe with in situ hybridization, and the distribution of HGV/GBV C related antigens in the liver tissues was detected with immunohistochemistry. Results HGV/GBV C RNA was detected in 58.33%, and HGV/GBV C NS5 antigen in 66.67% of 12 liver samples of hepatitis patients with HGV/GBV C infection alone. Positive staining signal of hybridization and HGV/GBV C NS5 antigen was only seen in the cytoplasm. Positive cells of hybridization was not related to hepatocyte degeneration, cholestasis, inflammatory cell infiltration, degrees of cell necrosis, etc. Eight cases of acute hepatitis and 4 of chronic hepatitis were diagnosed in 12 liver tissue samples. Conclusion It seems that HGV/GBV C does not damage hepatocytes directly, and HGV/GBV C related antigens may exist in hepatocytes; HGV/GBV C might exert its role as a target antigen in the course of liver injury.