潘生丁,凡拉帕米对逆转K562／ADM耐药性的研究

本研究结果提示:潘生丁、凡拉帕米均有增强ADM和VCR对K562和K562/ADM的作用,在ADM为0.01μg/ml时,对K562的抑制率为35.9％,当同时含有潘生丁6.25μg/ml～50.0μg/ml或凡拉帕米1.8μg/ml～14.4μg/ml时,使细胞的抑制率可分别再提高20.8％～34.50％和7.7％～29.5％,而对K562/ADM,潘生丁和凡拉帕米分别使细胞抑制率提高21.6％～70.9％和40.3％～61.1％。在VCR为0.1μg/ml时,对K562的抑制率为58.4％,当含有潘生丁或凡拉帕米时,抑制率可分别提高19.1％～24.9％和18.7％～22.6％,而对K562/ADM,在VCR8.0μg/ml时,抑制率为18.8％,当含有潘生丁或凡拉帕米时,抑制率可分别提高32.5％～45.1％和23.8％～35.4％。从上述结果可看出,潘生丁或凡拉帕米在逆转K562/ADM的耐药性是有益的。

A STUDY ON ANTI-DRUG-RESISTANCE ACTIVITY OF DIPYRIDAMOLE VERAPAMIL AGAINST K562/ADM CELLS

The result of this report showed that dipyridamole and verapamil canincrease the cytotoxic action of ADM and VCR on K562 and ADM resistant K562/ADM cell line. ADM at the dose of 0.01mg/ml in duced inhibitory rate of 35.9% on K562, When dipyridamole 6.25- 50.0u g/ml or verapamil 1.8-14.4u g/ml were added, the inhibitory rate increased by 20.8% - 34. 5% and 7.7%- 29.5% and the inhibitory rate on K562/ADM increased by 21.6%-70.9%and40.3%-61.1% .respectively. VCR at the dose of 6.1ug/ml induced inhibitory rate of 58.4% on K562 when dipyridamole or verapamil were added, the inhibitory rate on K562 increased by 19. l%-24.9% and 18.7%- 22.6% , respectively. VCR at the dose of 8.0ug/ml induced inhibitory rate of 18.8% , on K562/ADM but when dipyridamole or verapamil were added, the inhibitory rate can increase by 32.5%- 45.1% and 23.8% -35.4%, respectivery. These results, suggested that dipyridamole and verapamil are beneficial in reversing the drug-resistance of K562/ADM.