LC-MS测定埃博霉素B血浆蛋白结合率

目的 建立液相色谱串联质谱(LC-MS)测定方法并检测犬以及人体埃博霉素B的血浆蛋白结合率.方法 建立LC-MS联合检测方法,以犬和人体血浆为对象,结合甲衡透析法检测埃博霉素B的血浆蛋白结合率.结果 3种浓度(100、50和10 ng/mL)埃博霉素B与犬血浆蛋白结合率分别为(85.4％±2.5％)、(81.3％±2.2％)和(83.7％±3 4％),与人体血浆蛋白结合率分别为(74.4％±3 8％)、(81.0％±2.9％)和(75.6％±2.2％),且灵敏度高,重现性好,操作简单.结论 埃博霉素B血浆蛋白结合率高,LC-MS可用于埃博霉素B血浆蛋白结合率的检测.

Determination of the plasma protein binding rate of epothilone B by LC-MS

Objective To establish the method with liquid chromatography-mass spectrophotometer （LC-MS） and determine the protein binding rates of epothilone B in plasma of dog and human. Methods The equilibrium dialysis combined with LC-MS was performed to determine the drug concentration of epothilone B, and its protein binding rates in dog and human plasma. Results With different concentration of epothilone B including 100, 50 and 10 ng/mL, the protein binding rates of epothilone B in dog were （85.4%±2.5%）, （81.3%±2.2%） and （83.7%±3.4%）, and in human were（74.4%± 3.8%）, （81.0%± 2.9%） and （75.6%± 2.2%）, respectively. Conclusion Epothilone B has higher level in binding to plasma protein of dog and human. The LC-MS method can be usd to determine the drug concentration of epothilone B properly.