基于熵权-TOPSIS模型结合特征图谱的蜜麸枳壳原料产地优选及质量标准提升研究

目的 基于工业化生产条件进行蜜麸枳壳质量标准研究，并优选优势原料产地。方法 参照《中国药典》2020年版方法对18批不同产地来源的蜜麸枳壳进行显微、薄层色谱鉴别，测定水分、总灰分、醇溶性浸出物含量，通过色差仪测定饮片粉末色度，采用HPLC法测定其中柚皮苷和新橙皮苷含量，建立了特征图谱，采用聚类分析、正交偏最小二乘-判别分析（orthogonal partial least squares-discrimination analysis,OPLS-DA）等方法对结果进行分析，并利用熵权-逼近理想解排序法（technique for order preference by similarity to ideal solution,TOPSIS）评价各批次蜜麸枳壳质量。结果 蜜麸枳壳各指标结果均符合《中国药典》2020年版要求，通过建立的蜜麸枳壳特征图谱，得到15个共有峰，指认了6号峰为柚皮苷，7号峰为橙皮苷，8号峰为新橙皮苷，11号峰为橙皮素，12号峰为川陈皮素，13号峰为甜橙黄酮，15号峰为橘红素，聚类分析结果表明，18批蜜麸枳壳样品可聚为4类；通过OPLS-DA法筛选出新橙皮苷、峰10...

Preferential selection of raw material production areas and quality standard enhancement of honey bran Aurantii Fructus based on entropy weight-TOPSIS model combined with characteristic chromatogram

Objective To carry out research on the quality standard of honey bran Zhiqiao (Aurantii Fructus, hbAF) based on the production conditions of the enterprise, and preferr advantageous raw material production areas. Methods Referring to the method of 2020 edition of Chinese Pharmacopoeia, 18 batches of hbAF were identified by microscopy and thin-layer chromatography, and the contents of moisture, total ash, alcohol-soluble leachate were determined. The chromaticity of powder was determined by colorimeter, and the contents of naringin and neohesperidin were measured by HPLC. The characteristic chromatogram was established, and the results were analyzed by the methods of clustering analysis, orthogonal partial least squares- discrimination analysis (OPLS-DA), etc., and the entropy weight-TOPSIS method was utilized to evaluate the quality of hbAF of each batch. Results The results of all the indicators of hbAF met the requirements of the 2020 edition of Chinese Pharmacopoeia. Through the established characteristic chromatogram of hbAF, 15 common peaks were obtained and seven peaks were identified, and the clustering results showed that the 18 batches of samples could be clustered into four classes; Neohesperidin, peak 10, hesperidin, nobiletin, peak 9, and peak 5 were screened as the main differential components by the OPLS-DA method. The results of entropy weight-TOPSIS method showed that the comprehensive quality of hbAF of Hunan Yuanjiang was optimal, and it was recommended to use the local Aurantii Fructus as a raw material for production. Conclusion The methods are simple and easy for the quality control of hbAF. The entropy weight-TOPSIS model was used to evaluate the comprehensive quality of hbAF, and the reverse traceability to screen the advantageous raw material sources, which can provide a reference for the quality improvement of hbAF in the process of industrialized production of hbAF.