OxyR contributes to the virulence of a Clonal Group A Escherichia coli strain (O17:K+:H18) in animal models of urinary tract infection,subcutaneous infection,and systemic sepsis |
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| Affiliation: | 1. Veterans Affairs Medical Center, 1 Veterans Drive, Minneapolis, MN 55417, USA;2. University of Minnesota, 420 Delaware St. S.E., Minneapolis, MN 55455, USA;3. Veterans Administration Western New York Healthcare System, 3495 Bailey Ave, Buffalo, NY 14215, USA;4. Department of Medicine, The University at Buffalo-State University of New York, 3435 Main Street, Buffalo, NY 14214, USA;5. Department of Microbiology and Immunology, The University at Buffalo-State University of New York, 3435 Main Street, Buffalo, NY 14214, USA;6. The Witebsky Center for Microbial Pathogenesis, The University at Buffalo-State University of New York, 3435 Main Street, Buffalo, NY 14214, USA;7. Department of Medicine, University of Washington, 1959 NE Pacific Street, Seattle, WA 98195, USA;1. Nucleo de Medicina Tropical, Universidade Federal do Para, Belem-Pa, Brazil;2. Centro de Ciências Biologicas e da Saude, Universidade do Estado do Para, Belem-Pa, Brazil;1. FES Iztacala, Universidad Nacional Autónoma de México, Estado de Mexico, Mexico;2. Instituto Mexicano del Seguro Social, Mexico;1. Eudowood Division of Pediatric Respiratory Sciences, Johns Hopkins School of Medicine, Baltimore, MD, United States;2. W. Harry Feinstone Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Baltimore, MD, United States;3. Division of Pulmonary and Critical Care Medicine, Department of Medicine, Johns Hopkins School of Medicine, United States;4. Northwestern University Feinberg School of Medicine, Medicine, Chicago, IL, United States;1. National Cancer Institute, Cairo University, Giza, Egypt;2. Parasitology Department, Faulty of Medicine, Mansoura University, Mansoura, Egypt;3. Hygiene Department, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt;4. Virology Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni Suef 62511, Egypt;5. Microbiology Department, Virology Division, College of Medicine, Taif University, Al-Taif 21944, Saudi Arabia;1. Toxicological Biochemistry of Graduate Program, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil;2. Department of Microbiology and Parasitology, UFSM, Santa Maria, RS, Brazil;3. Graduate Program in Pharmacology, UFSM, Santa Maria, Brazil;4. Lutheran University of Brazil (ULBRA), Santa Maria, RS, Brazil;5. Department of Large Animal, UFSM, Santa Maria, RS, Brazil;6. Department of Chemistry, Federal University of Santa Maria, Brazil;7. Department of Animal Science, Universidade do Estado de Santa Catarina (UDESC), Chapecó, SC, Brazil;1. Department of Parasitology, Microbiology and Immunology, Institute of Biological Sciences, Federal University of Juiz de Fora, 36036-900 Juiz de Fora, Minas Gerais, Brazil;2. Department of Biology, Institute of Biological Sciences, Federal University of Juiz de Fora, 36036-900 Juiz de Fora, Minas Gerais, Brazil;3. Department of Morphology, Institute of Biological Sciences, Federal University of Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil |
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| Abstract: | The oxidative stress response regulator OxyR was assessed as both a urinary and extra-urinary virulence factor in Escherichia coli strain UCB34 (O17:K+:H18), a representative of the emergent Clonal Group A (CGA). Compared to UCB34, the isogenic oxyR mutant exhibited increased H2O2 sensitivity, indistinguishable in vitro growth, and attenuated virulence in rodent models of urinary tract, subcutaneous infection, and systemic sepsis. Complemented mutants showed virulence levels comparable to parent strains in all models. These findings uniquely fulfill molecular Koch's postulates for a putative virulence factor of CGA, provide experimental evidence of an extra-urinary virulence promoting trait in CGA, and document a role for OxyR in local and systemic extra-urinary E. coli infections. |
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| Keywords: | Virulence OxyR Urinary tract infections Extra-urinary infections Clonal Group A |
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