|
|||||
|
|
| 白花败酱HPLC指纹图谱研究 | |
| 引用本文： | 张一芳,周淑琴,吴国忠. 白花败酱HPLC指纹图谱研究[J]. 中国药房, 2011, 0(3): 242-243 |
| 作者姓名： | 张一芳 周淑琴 吴国忠 |
| 作者单位： | 上海医药高等专科学校药学系,上海市201318 |
| 基金项目： | 上海高校选拔培养优秀青年教师科研专项基金资助项目（yyz-07002） |
| 摘 要： | 目的:建立白花败酱高效液相色谱(HPLC)指纹图谱,为白花败酱的品种鉴别和质量控制提供参考依据。方法:色谱柱为Kromasil-C18(250mm×4.6mm,5μm),流动相为乙腈-0.1%磷酸(梯度洗脱),流速为0.8mL·min-1,柱温为25℃,检测波长为320nm。结果:确定由15个峰构成白花败酱的指纹特征,10批样品相似度较好。结论:本方法精密度、稳定性、重复性良好,可为白花败酱的鉴别和质量评价提供参考。 |
| 关 键 词： | 白花败酱 指纹图谱 高效液相色谱法 |
Study on HPLC Fingerprints of Patrinia villosa |
|
| ZHANG Yi-fang,ZHOU Shu-qin,WU Guo-zhong. Study on HPLC Fingerprints of Patrinia villosa[J]. China Pharmacy, 2011, 0(3): 242-243 | |
| Authors: | ZHANG Yi-fang ZHOU Shu-qin WU Guo-zhong |
| Affiliation: | (Dept.of Pharmacy,Shanghai Institute of Health Sciences,Shanghai 201318,China) |
| Abstract: | OBJECTIVE：To establish HPLC fingerprints of Patrinia villosa,and to provide reference for identification and quality control of it.METHODS：The chromatographic column was Kromasil-C18（250 mm×4.6 mm,5μm）and the mobile phase consisted of acetonitrile-0.1% phosphoric acid at flow rate of 0.8 mL·min-1.The column temperature was 25℃ and the detection wavelength was set at 320 nm.RESULTS：15 peaks were identified as the characteristic finger prints of P.villosa.10 batches of samples had high similarity.CONCLUSION：The method has a good precision,stability and repeatability.The established fingerprints can be used for the quality control and species identification of P.villosa. |
| Keywords: | Patrinia villosa Fingerprints HPLC |
| 本文献已被 维普 等数据库收录！ | |