狭基线纹香茶菜水提部位对免疫肝损伤小鼠NF-kB蛋白表达水平及核转位的影响

目的：研究狭基线纹香茶菜水提部位对免疫肝损伤小鼠核因子KB（NF—KB）蛋白表达水平及核转位的影响。方法：小鼠尾静脉注射刀豆蛋白A（ConA）25mg·kg。复制肝损伤模型，免疫组化法检测NF-kB在肝组织的表达，以及NF．KB从细胞浆向细胞核转位的情况。结果：复制模型4、8h后小鼠NF．KB蛋白表达显著增加（P〈0．01），吡咯烷二硫代氨基甲酸盐（PDTC）和狭基线纹香荼菜水提部位9．2g·kg。在4、8h均可显著降低模型小鼠NF．1（El的蛋白表达量（P〈O．01）。ConA能显著促进NF—KB自细胞浆向细胞核转位（P〈O．01）。PDTC和狭基线纹香茶菜提取物9．2g·kg-1。能显著减少NF-kB在细胞核表达，使其在细胞浆表达显著增多（P〈0．01）。结论：狭基线纹香茶菜可抑制免疫肝损伤小鼠NF-kB蛋白的表达，并抑制NF-kB的核转位。狭基线纹香茶菜对抗免疫肝损伤的作用机制可能与其抑制核NF-kB的激活有关。

Effect of Water-soluble Extract from Isodon lophanthoides on the Expression of NF-KB Protein and Nuclear Translocation in Mice with Immune Hepatic Injury

OBJECTIVE： To study the effect of water-soluble extract from Isodon lophanthoides on the expression of NF-kB protein and nuclear translocation in mice with immune hepatic injury. METHODS： Hepatic injury model was induced by tail intrave- nous injection of Con A 25 rag. kg-l. The expression of NF-~：B protein and nuclear translocation in liver tissue were detected by im- munohistochemistry examination. RESULTS： The expression of NF-kB protein increased significantly 4 h and 8 h after modeling （P〈0.01）. PDTC and water-soluble extract （9.2 g·kg-1） can inhibit the expression of NF-kB protein（P〈0.01）. ConA can improve that NF-KB translocated from cytoplasm into nucleus（P〈：0.01）. PDTC and water-soluble extract （9.2 g. kg-1） reduced the expression of NF-kB protein in nucleus, and increased that in cytoplasm. CONCLUSION：1. lophanthoides can down-regulate the expres- sion of NF-kB protein and inhibit nuclear translocation. Inhibiting effect of 1. lophanthoides on immune hepatic injury is associated with the inhibition of the activation of NF-kB .