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| 幽门螺杆菌尿素酶B亚单位核酸疫苗的发酵、纯化及鉴定 | |
| 引用本文： | 曾韦锟,邹全明,朱永红. 幽门螺杆菌尿素酶B亚单位核酸疫苗的发酵、纯化及鉴定[J]. 中华消化外科杂志, 2005, 4(4): 275-278 |
| 作者姓名： | 曾韦锟 邹全明 朱永红 |
| 作者单位： | 1. 400037,第三军医大学新桥医院检验科 2. 400038,第三军医大学生物医药中试研究基地暨临床微生物学及免疫学教研室 |
| 基金项目： | 国家“863”计划资助项目(No.2001AA215161) |
| 摘 要： | 目的大规模发酵、纯化幽门螺杆菌尿素酶B亚单位(ureB)基因疫苗,并对纯化后的疫苗进行鉴定。方法大规模发酵DH5α(pT ureB),收获的细菌经碱裂解后,用两步离子交换层析方法分离、纯化疫苗,通过紫外分光光度计、酶切、体外转染等对疫苗进行鉴定。结果发酵幽门螺杆菌ureB核酸疫苗,并经离子交换层析方法纯化获得了高质量的疫苗。结论纯化的ureBDNA疫苗为进一步的免疫实验奠定了基础。 |
| 关 键 词： | Helicobacterpylori DNA疫苗 纯化 |
| 文章编号： | 1671-4555(2005)04-0275-04 |
| 修稿时间： | 2005-02-05 |
Fermentation, purification and identification of ureB gene vaccine of H.pylori |
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| Zeng Weikun,ZOU Quanming,Zhu Yonghong. Fermentation, purification and identification of ureB gene vaccine of H.pylori[J]. Chinese Journal of Digestive Surgery, 2005, 4(4): 275-278 | |
| Authors: | Zeng Weikun ZOU Quanming Zhu Yonghong |
| Abstract: | Objective To ferment, purify and identify ureB gene vaccine of H. pylori. (Methods) Fermented DH5a (pT-ureB) were lysed using the alkaline method, and lysate was dealed with two-step anion-exchange chromatography. Then the purified gene vaccine was further analyzed by spectrophotometer, restriction enzyme digestion and transfection. Results The ureB gene vaccine were fermented successfully, and the purified gene vaccine was of high quality. Conclusions Purified ureB gene vaccine lays the foundations for further experiments in vivo. |
| Keywords: | Helicobacter pylori DNA vaccine purification |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |