|
|||||
|
|
| 药用灵芝遗传多样性的AFLP分析 | |
| 引用本文： | 郑林用,贾定洪,罗霞,杨志荣,. 药用灵芝遗传多样性的AFLP分析[J]. 中国中药杂志, 2007, 32(17): 1733-1736 |
| 作者姓名： | 郑林用 贾定洪 罗霞 杨志荣 |
| 作者单位： | 1. 四川大学,生命科学院,四川,成都,610064;四川省农业科学院,土壤肥料研究所,四川,成都,610066 2. 四川省农业科学院,土壤肥料研究所,四川,成都,610066 3. 四川大学,生命科学院,四川,成都,610064;四川省中药研究所,四川,成都,610041 4. 四川大学,生命科学院,四川,成都,610064 |
| 摘 要： | 目的：分析不同药用灵芝栽培菌株的遗传差异,为灵芝道地性研究、种质资源鉴定、亲缘关系研究、品种选育和引种栽培提供分子生物学依据。方法：以24个来源不同的灵芝及紫芝栽培菌株和野生菌株为材料,采用扩增酶切片断多态性AFLP方法,用NTSYSpc 2.1软件进行UPGMA法聚类分析,构建遗传系统树。结果：筛选14对引物,共扩增出177条多态性谱带。聚类结果表明利用AFLP技术可将全部供试材料区分开。结论：药用灵芝栽培菌株在分子水平上存在较大差异,并在相似系数0.676水平上聚为赤芝群和紫芝群。 |
| 关 键 词： | 灵芝 遗传多样性 聚类分析 |
| 文章编号： | 1001-5302（2007）17-1733-04 |
| 收稿时间： | 2006-07-10 |
| 修稿时间： | 2006-07-10 |
AFLP analysis for genetic diversity of Ganoderma |
|
| ZHENG Lin-yong;; JIA Ding-hong; LUO Xia;; YANG Zhi-rong. AFLP analysis for genetic diversity of Ganoderma[J]. China Journal of Chinese Materia Medica, 2007, 32(17): 1733-1736 | |
| Authors: | ZHENG Lin-yong JIA Ding-hong LUO Xia YANG Zhi-rong |
| Affiliation: | 1. Key Laboratory of Biological Resource and Ecological Environment of the Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, China; 2. Soil and Fertilizer Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China; 3. Sichuan Institute of Chinese Material Medica, Chengdu 610041, China |
| Abstract: | Objective: To investigate the genetic diversity of Ganoderma cultivars provided for the genuineness study, germplasm resource identification, genetic relationship study, breeding, introduction and cultivante of Ganoderma strains. Method: With the software of NTSYSpc 2.1, 24 materials, of G.lucidum and G.sinense, were studied using AFLP to construct the dendrogram. Result: There were 177 polymorphic bands with 14 primer combinations. And all materials could be identified with AFLP. Conclusion: There actually existed much genetic diversity at the molecular level among the germplasm resources of Ganoderma strains, and all the strains were clustered into G. lucidum group and G.sinense group at the similarity coefficient 0.676. |
| Keywords: | AFLP |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |