| 皮质酮对大鼠肝再生过程中鸟氨酸脱羧酶的抑制 |
| |
| 引用本文： | 马建敏,和俊涛,索世英,宁黔冀,徐存栓. 皮质酮对大鼠肝再生过程中鸟氨酸脱羧酶的抑制[J]. 解剖学报, 2008, 39(5): 693-698 |
| |
| 作者姓名： | 马建敏 和俊涛 索世英 宁黔冀 徐存栓 |
| |
| 作者单位： | 河南师范大学生命科学学院;河南师范大学生命科学学院;省部共建细胞分化调控国家重点实验室,河南,新乡,453007;省部共建细胞分化调控国家重点实验室,河南,新乡,453007 |
| |
| 摘 要： | 目的 研究大鼠体内主要的糖皮质激素--皮质酮对部分肝切除(PH)诱导的再生肝鸟氨酸脱羧酶(ODC)基因表达及酶活性的影响.方法 乙醚麻醉大鼠,于PH前3d行双侧肾上腺切除术及假手术,皮质酮悬浮于芝麻油中皮下注射玄肾上腺鼠.用RT-PCR、Western blotting及高效液相色谱法(HPLC)分别测定ODC mRNA、ODC蛋白及酶活性.结果 与再生肝相比,完整肝中ODC mRNA、蛋白及其酶活性水平较低.PH后所有组(每组n=6)中ODCmRNA水平均显著升高,5h达到峰值.7h前mRNA含量由高到低依次为去肾上腺组、去肾上腺假手术组(即对照组)、10和40ms/kg体重皮质酮处理组.去肾上腺组(n=13,下同)的ODC蛋白量高于对照组.10mg/kg体重皮质酮处理,在PH后0～24h下降,40mg/kg皮质酬处理组最低.PH后,所有组(每组n=6)ODC活性迅速升高,6h达到峰值;皮质酮处理后活性在6h下降显著,40ms/kg体重皮质酮处理组活性最低.结论 皮质酮处理后,大鼠完整肝及再生肝中ODC mRNA及蛋白水平呈剂量依赖性下降;ODC活性变化的部分原因是由于ODC mRNA及其蛋白量的改变.
|
| 关 键 词： | 皮质酮 肝再生 鸟氨酸脱羧酶 RT-PCR Western blotting 高效液相色谱法 大鼠 |
| 收稿时间： | 2007-05-20 |
| 修稿时间： | 2008-03-14 |
INHIBITION OF GLUCOCORTICOID ON ORNITHINE DECARBOXYLASE DURING RAT LIVER REGENERATION |
| |
| MA Jian-min,HE Jun-tao,SUO Shi-ying,NING Qian-ji,XU Cun-shuan. INHIBITION OF GLUCOCORTICOID ON ORNITHINE DECARBOXYLASE DURING RAT LIVER REGENERATION[J]. Acta Anatomica Sinica, 2008, 39(5): 693-698 |
| |
| Authors: | MA Jian-min HE Jun-tao SUO Shi-ying NING Qian-ji XU Cun-shuan |
| |
| Affiliation: | 1.College of Life Science, He′nan Normal University； 2.State Key Laboratory of the Cell Differentiation and Regulation of He′nan Province and Ministry of Science and Technology, He′nan Xinxiang 453007, China |
| |
| Abstract: | Objective The regulation of ornithine decarboxylase(ODC) gene expression and enzyme activity by corticosterone,the main glucocorticoid in rat,during rat liver regeneration induced by partial hepatectomy(PH) was evaluated.Methods Bilateral adrenalectomies(ADX) and sham-ADX were performed on ether-anesthetized rats 3 days before PH.Corticosterone in sesame oil was injected subcutaneously to adrenalectomied rats.ODC mRNA,ODC protein and enzyme activity were detected by RT-PCR,Western blotting and high performa... |
| |
| Keywords: | RT-PCR Western blotting Corticosterone Liver regeneration Ornithine decarboxylase RT-PCR Western blotting High performanceliquid chromatography Rat |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《解剖学报》浏览原始摘要信息 |
|
点击此处可从《解剖学报》下载全文 |
|
