锌金属硫蛋白对镉致肝肾损伤的修复作用研究

目的：探讨锌金属硫蛋白（Zn-MT）对镉所导致的肝肾毒性的修复作用。方法：以小鼠为研究对象，建立镉中毒动物模型，然后经口给予不同剂量的Zn-MT,测定并分析肝、肾组织中脂质过氧化代谢产物丙二醛（MDA）水平、超氧化物歧化酶（SOD）、谷胱甘肽过氧化酶（GSH－Px）活性及电镜下观察肝肾组织形态学变化。结果：Zn-MT可明显降低肝、肾组织中MDA水平，使肝、肾组织中SOD、GSH－Px活力有一定程度的恢复，且上述作用呈明显的剂量－反应关系；电镜下观察到给予Zn-MT后，肝、肾组织形态学病变得到一定程度的恢复。结论：Zn-MT可对镉所致的肝肾组织脂质过氧化损伤起到一定的修复作用。

Study on Renovating Effect of Zinc - metallothionein on Cadmium - induced Oxidative in Liver and Kidney

ObjectiveTo study the renovating effect of zinc-metallothionein to cadmium-induced oxidative in liver and kidney.MethodsKunming mice were acted as tested objects to establish the cadmium-poisoning laboratory animal models,then animals were treated with zinc-metallothionein(Zn-MT).Following indexes were assayed,including the contents of malony diadehyde(MDA),the activities of superoxide dismutase(SOD) and glutathione peroxidase(GSH-Px) in liver and kidney,observing the ultrastructure of liver and kidney under transmission electron microscope.ResultsThe contents of DMA in liver and kidney of animals which administrated with Zn-MT decreased and the suppressed activities of antioxidase(SOD GSH-Px) renovated.The pathomorphologic damages of liver and kidney induced by cadmium were renovated to some extent after administrated with Zn-MT.ConclusionZn-MT had renovating effect on cadmium-induced oxidative damage in liver and kidney.