白细胞介素-2基因转导人胃癌细胞的实验研究

为研究白细胞介素－２（ＩＬ－２）基因转导人胃癌细胞的可行性和有效性，用真核表达载体，将人ＩＬ－２ｃＤＮＡ转导入人胃癌细胞株ＳＧＣ－７９０１细胞表达，并观察了基因转导和表达对胃癌细胞体外生长特性和ＬＡＫ细胞的肿瘤杀伤作用的影响，以及基因转导细胞的体内致瘤性。结果表明，人ＩＬ－２ｃＤＮＡ可以转导入人胃癌细胞，并表达有活性的ＩＬ－２，基因转导不改变胃癌细胞的体外生长特性，并能增强ＬＡＫ细胞的肿瘤杀伤作用，降低或消除胃癌细胞的体内致瘤性。本研究结果提示：ＩＬ－２基因转导胃癌细胞可能是防治胃癌的有效方法。

TRANSDUCTION OF THE IL 2 GENE INTO HUMAN GASTRIC CANCER CELL: AN EXPERIMENTAL STUDY

We evaluated the possibility of inducing a productive transfer of the IL 2 gene into human gastic cancer cells (GCC) and assessed the phenotypic and proliferative changes generated in the nude mice. The plasmid vector (BMGNeo IL 2) carrying the human IL 2 gene was used to transduce the SGC 7901 GCC line by the lipofectin reagent. The IL 2 gene was analyzed by Southern blot and productive IL 2 release using IL 2 dependent CTLL. Cytotoxicity of LAK cells was tested by MTT colorimetry. The kinetics of in vitro growth and proliferation of parental and engineered cells were also measured. Parental and IL 2 gene transduced GCC were injected into nude mice. The tumorigenic potential of IL 2 gene transfected GCC was evaluated by examining their in vivo growth in nude mice. The productive insertion of the IL 2 gene was achieved in SGC 7901. The amounts of IL 2 constitutively released by the engineered neoplastic cells ranged from 20 to 131 U/ml of IL 2 produced from 10 6 cells in 24 hours. Transduction of GCC with IL 2 gene did not modify the morphology and growth rate. IL 2 gene transfected cells demonstrated increased susceptibility to cell killing by LAK cells. IL 2 producing cells lost their tumorigenicity as evidenced by failure to grow in nude mice. The results demonstrate that IL 2 gene can be productively trasduced into human gastric cancer cells without modifying their morphology and growth rate and this transduction leads to reduced or abrogated in vivo tumorigenic potential.