Elf-1和VEGF在非小细胞肺癌组织中的表达及意义

背景与目的：血管内皮生长因子（vascular endothelial growth factor，VEGF）促进肿瘤血管生成的作用与Ets家族有关，Ets家族中对Elf-1的研究较少。本研究检测非小细胞肺癌（non—small cell lung cancer，NSCLC）组织中转录因子Elf-1和VEGF的表达，探讨其与NSCLC临床病理特征的关系。方法：制备含69例NSCLC组织和6例正常肺组织的组织芯片，采用免疫组化Power Vision-9000法检测Elf-1和VEGF蛋白的表达水平。结果：Elf-1和VEGF在正常肺组织中的表达均为阴性，在NSCLC组织中表达的阳性率分别为72．46％和63．77％，其表达水平与肿瘤细胞分化程度、淋巴结转移、临床分期和术后生存期有关（P〈0．01）。Kaplan—Meier生存分析表明二者的过表达均与患者的生存率有关，阳性表达的患者生存率明显低于阴性者（P〈0．01）。在NSCLC中，Elf-1的表达与VEGF的表达呈正相关（r=0．702，P〈0．01）。结论：Elf-1和VEGF在NSCLC组织中的过表达与肿瘤的分化程度、转移和预后均有关，联合检测二者的表达水平可作为判定NSCLC恶性生物学行为的参考指标。

Expression and significance of Elf-1 and vascular endothelial growth factor in non-small cell lung cancer

Background and Objective： The roles of vascular endothelial growth factor （VEGF） in tumor angiogenesis is related with Ets family. Elf-1, a member of Ets family, has seldom been studied. This study aimed to investigate the expression of Elf-1 and VEGF in non-small cell lung cancer （NSCLC）, and explore their correlations to clinicopathologic features of NSCLC. Methods： Tissue microarray containing 69 specimens of NSCLC and six specimens of normal lung tissues was constructed. The expression of Elf-1 and VEGF was detected by PowerVisionTU-9000 immunohistochemistry. Results= Elf-1 and VEGF were not detected in all normal tissues; the positive rates of Elf-1 and VFGF were 72.46% and 63.77% in NSCLC, respectively. The expression levels of both Elf-1 and VEGF were significantly related with tumor differentiation, lymphatic metastasis, clinical stage, and postoperative survival time （all P 〈 0.01 ）. Overexpression of them was related with poor prognosis： the survival rates were significantly lower in positive patients than in negative patients （both P 〈 0.01 ）. Elf-1 expression was positively correlated to VEGF expression （r = 0.702, P 〈 0.01 ）. Conclusions： The expression of Elf-1 and VEGF in NSCLC is related to differentiation, lymphatic metastasis, clinical stage and prognosis. Detecting their expression in combination can help to predict the malignant behavior of NSCLC.