鼻腔鼻窦内翻性乳头状瘤中MMP-9、PCNA的表达及临床意义

目的：探讨MMP-9、PCNA在鼻内翻性乳头状瘤(SNIP)发病及其复发和恶变中的作用。方法：选取40例SNIP及10例鼻腔鼻窦鳞状细胞癌（SCC）组织蜡块，全部标本分为4组，即良性SNIP组、复发SNIP组、恶变SNIP组及SCC组。利用免疫组织化学染色技术检测MMP-9、PCNA在各组中的表达情况。结果：MMP-9、PCNA在良性SNIP组、复发SNIP组、恶变SNIP组及SCC组中的表达逐渐增强，经秩和检验两两比较，显示MMP-9的表达在良性SNIP组与复发SNIP组，良性SNIP组与恶变SNIP组，良性SNIP组与SCC组之间均有显著性差异，复发SNIP组与恶变SNIP组，复发SNIP组与SCC组，恶变SNIP组与SCC组之间无明显差异；PCNA的表达在各组间差异无显著性。结论：MMP-9、PCNA的表达参与SNIP的发病，并在其复发和恶变中起重要作用，可能是SNIP复发和恶变的早期事件之一。

Expression of MMP-9 and PCNA in sinonasal inverted papilloma and the clinical significance

Objective:To explore the function of MMP-9 and PCNA in the occurrence,recurrence and malignancy of the sinonasal inverted papilloma(SNIP).Methods: Forty specimens with SNIP and ten specimens with squamous cell carcinoma(SCC) were selected for this study.All the specimens were divided into four groups: the SNIP group,the recurrent SNIP group,the SNIP with carcinoma group,and the invasive squamous cell carcinoma(SCC) group.The expression of MMP-9 and PCNA was determined by using an immunohistochemistry method.The results were analyzed by a quantitative immunohistochemical analysis.Results: Expression of MMP-9 and PCNA was significantly increased in the SNIP group,the recurrent SNIP group,the SNIP with carcinoma group,and the SCC group.According to the rank test,the expression of MMP-9 was significantly different between the SNIP group and other groups.But between recurrent SNIP and SNIP with carcinoma,recurrent SNIP and SCC,SNIP with carcinoma and SCC,there were no prominent differences.The expression of PCNA was not significantly different between any two groups.Conclusion: The expression of MMP-9 and PCNA is involved in the mechanism of the occurrence of SNIP and plays an important role in the recurrence and malignancy of SNIP.The over expression of MMP-9 and PCNA may be associated with early events in the recurrence tendency and carcinogenesis/transformation to SNIP.