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| BALB/c 小鼠小肠乳糖酶活性双糖酶法测定条件的优化 | |
| 引用本文： | 何伟,黄磊,吕斌,黄承钰. BALB/c 小鼠小肠乳糖酶活性双糖酶法测定条件的优化[J]. 郑州大学学报(医学版), 2005, 40(3): 405-408 |
| 作者姓名： | 何伟 黄磊 吕斌 黄承钰 |
| 作者单位： | 1. 四川大学华西公共卫生学院营养与食品卫生学教研室,成都,610041;河南省医学科学研究所,郑州,450052 2. 四川大学华西公共卫生学院营养与食品卫生学教研室,成都,610041 3. 郑州大学2003级硕士生,郑州,450052 |
| 基金项目： | 国家自然科学基金资助项目 30271126 |
| 摘 要： | 目的建立一种方便、准确测定小鼠小肠乳糖酶活性的方法.方法采用不同pH的底物溶液与肠黏膜提取物反应,计算相应的酶活性;取不同反应时间(45 min,60 min,75 min),不同显色时间(45 min,60 min,75 min)和不同显色剂用量(2.0 ml,2.5 ml,3.0 ml)按L9(34)正交实验设计方法,测定不同条件下的底物溶液与肠黏膜反应的A值作为正交实验的评分标准.结果与结论由正交实验得出,该方法的最佳反应条件为pH 4.6,水浴75min,显色时间45 min,显色剂用量3.0 ml;批内酶活性(0.029±0.003)U/ml,CV=2.49%,批间酶活性(0.032±0.004)U/ml,CV=4.19%.该方法具有很好的重复性和可靠性. |
| 关 键 词： | 乳糖酶 BALB／c小鼠 小肠 |
| 修稿时间： | 2005-03-07 |
A study on the method of lactase activity determination in small intestine of BALB/c mouse |
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| HE Wei,HUANG Lei,LV Bin,HUANG Chengyu. A study on the method of lactase activity determination in small intestine of BALB/c mouse[J]. Journal of Zhengzhou University: Med Sci, 2005, 40(3): 405-408 | |
| Authors: | HE Wei HUANG Lei LV Bin HUANG Chengyu |
| Affiliation: | HE Wei 1,2),HUANG Lei 1),LV Bin 3),HUANG Chengyu 1) 1)Department of Nutrition and Food Hygiene,Huaxi Public School of Health,Sichuan University,Chengdu 610041 2)Henan Institute of Medical Sciences,Zhengzhou 450052 3)Postgraduate of Grade 2003,Zhengzhou University,Zhengzhou 450052 |
| Abstract: | Aim: To optimize the conditions of determining the activity of lactase in the small intestine of mice.Methods: The mucosa extract of small intestine were mixed with substrate solutions of different pH, then the lactase activities of samples were calculated;different reaction time(45 min,60 min,75 min),developing time(45 min,60 min,75 min),and dosage of developer(2.0 ml,2.5 ml,3.0 ml) were applied in orthogonal experiment,and absorbency at 420 nm was determined. Results and conclusion: The best conditions of this method were: pH 4.6, water bath for 75 min, color developing for 45 min, and 3.0 ml of developer. The method is precise and convenient with better replication and liability. |
| Keywords: | lactase BALB/c mouse small intestine |
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