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抗内毒素类脂A单克隆抗体对烧伤脓毒症大鼠的保护作用
引用本文:肖红,府伟灵,肖光夏. 抗内毒素类脂A单克隆抗体对烧伤脓毒症大鼠的保护作用[J]. 中华整形外科杂志, 1996, 0(3)
作者姓名:肖红  府伟灵  肖光夏
作者单位:第三军医大学西南医院烧伤研究所
摘    要:
采用我所研究制备的抗内毒素类脂A单克隆抗体(mAb)观察了其对攻菌后动物的保护作用,并采用30%Ⅲ度TBSA烧伤大鼠模型注射内毒素,模拟临床烧伤脓毒症,旨在为临床防治提供理论依据。实验动物分烧毒组、治疗组及对照组,于伤后不同时相点观察血浆LPS、TNF的动态变化,同时作内脏的光镜及电镜形态学检查。结果表明:mAb除在体外可与多种G-杆菌及纯化的内毒素反应外,细菌攻击前后注入mAb均能有效地提高感染动物的存活率,并可明显降低模拟烧伤脓毒症大鼠血中内毒素,减少TNF的分泌。光、电镜观察证实,可减轻机体的损害效应。提示:抗类脂A单克隆抗体是一种有效的治疗烧伤脓毒症的免疫制剂。

关 键 词:烧伤,感染,内毒素,类脂A,单克隆抗体

Protective effect of lipid A monoclonal antibody against burn sepsis in rats
Xiao Hong, Fu Wriling,Xiao Guangxia.. Protective effect of lipid A monoclonal antibody against burn sepsis in rats[J]. Chinese journal of plastic surgery, 1996, 0(3)
Authors:Xiao Hong   Fu Wriling  Xiao Guangxia.
Abstract:
A lipid A monoclonal antibody(mAb) was prepared and it was used to study its protective effect against burn sepsis. Wistar rats were inflicted with 30% TBSA third degress burn, and they were given LPS to mimic early sepsis after burn. The rats were divided randomly into burn with LPS,monoclonal antibody treatment, and control groups. The levels of endotoxin, tumor necrosis factor, light and electron microscopic studies of the morphological changes in the liver were studied. The results showed that the anti-lipid A monoclonal antibody demonstrated capacity to cross-react with several Gram-negative bacteria and their endotoxins. The mAb improved the survival rate of rats and decreased the levels of endotoxin and TNF as well as the liver damage significantly.
Keywords:Burn  Sepsis  LPS  Lipid A  Monoclonal antibody
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